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Characterization of Human CD133+Cells in Biocompatible Poly(l-lactic acid) Electrospun Nano-Fiber Scaffolds
KTH, Skolan för bioteknologi (BIO), Industriell bioteknologi.
KTH, Skolan för bioteknologi (BIO), Industriell bioteknologi.
KTH, Skolan för bioteknologi (BIO), Industriell bioteknologi.ORCID-id: 0000-0002-0841-8845
KTH, Skolan för bioteknologi (BIO), Industriell bioteknologi.
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2016 (Engelska)Ingår i: Journal of Biomaterials and Tissue Engineering, ISSN 2157-9083, E-ISSN 2157-9091, Vol. 6, nr 12, s. 959-966Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

CD133+ cells are potential myogenic progenitors for skeletal muscle regeneration to treat muscular dystrophies. The proliferation of human CD133+ stem cells was studied for 14 days in 3D biomimetic electrospun poly-L-lactic acid (PLLA) nano-fiber scaffolds. Additionally, the myogenic differentiation of the cells was studied during the last 7 days of the culture period. The cells were homogeneously distributed in the 3D scaffolds while colony formation and myotube formation occurred in 2D. After a lag phase due to lower initial cell attachment and an adaptation period, the cell growth rate in 3D was comparable to 2D after 7 and 14 days of culture. The expression of the stem cell (SC) marker PAX7 was 1.5-fold higher in 3D than 2D while the differentiation markers MyoG, Desmin and MyoD were only slightly changed (or remain unchanged) in 3D but strongly increased in 2D (12.6, 3.9, and 7.9-fold), and the myotube formation observed in 2D was absent in 3D. The marker expression during proliferation and differentiation, together with the absence of myotubes in 3D, indicates a better maintenance of stemness in 3D PLLA and stronger tendency for spontaneous differentiation in 2D culture. This makes 3D PLLA a promising biomaterial for the expansion of functional CD133+ cells.

Ort, förlag, år, upplaga, sidor
American Scientific Publishers, 2016. Vol. 6, nr 12, s. 959-966
Nyckelord [en]
Myogenic Progenitor Cell, CD133+Cells, Myogenic Differentiation, 3D Cell Culturing, Electrospun Biodegradable Nano-Fiber Scaffold
Nationell ämneskategori
Biologiska vetenskaper
Identifikatorer
URN: urn:nbn:se:kth:diva-198952DOI: 10.1166/jbt.2016.1531ISI: 000387148500005Scopus ID: 2-s2.0-84998636398OAI: oai:DiVA.org:kth-198952DiVA, id: diva2:1065112
Forskningsfinansiär
EU, FP7, Sjunde ramprogrammet
Anmärkning

QC 20170113

Tillgänglig från: 2017-01-13 Skapad: 2016-12-22 Senast uppdaterad: 2017-11-29Bibliografiskt granskad

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Gillner, KarinZhang, YeÅstrand, CarolinaShokri, AtefehChotteau, Véronique

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Al-Khalili, LubnaGillner, KarinZhang, YeÅstrand, CarolinaShokri, AtefehChotteau, Véronique
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Journal of Biomaterials and Tissue Engineering
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