Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
MicroBubble Activated Acoustic Cell Sorting: BAACS
KTH, Skolan för bioteknologi (BIO), Proteomik och nanobioteknologi. KTH, Centra, Science for Life Laboratory, SciLifeLab. mafaridi@kth.se. (Clinical Microfluidics)ORCID-id: 0000-0003-1176-0905
KTH, Skolan för bioteknologi (BIO), Proteomik och nanobioteknologi. KTH, Centra, Science for Life Laboratory, SciLifeLab. (Clinical Microfluidics)ORCID-id: 0000-0001-5199-0663
KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik.ORCID-id: 0000-0003-0064-0086
KTH, Skolan för teknik och hälsa (STH), Medicinsk teknik, Medicinsk bildteknik.ORCID-id: 0000-0002-3699-396X
Visa övriga samt affilieringar
2017 (Engelska)Ingår i: Biomedical microdevices (Print), ISSN 1387-2176, E-ISSN 1572-8781, Vol. 19, nr 2, artikel-id 23Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Acoustophoresis, the ability to acoustically manipulate particles and cells inside a microfluidic channel, is a critical enabling technology for cell-sorting applications. However, one of the major impediments for routine use of acoustophoresis at clinical laboratory has been the reliance on the inherent physical properties of cells for separation. Here, we present a microfluidic-based microBubble-Activated Acoustic Cell Sorting (BAACS) method that rely on the specific binding of target cells to microbubbles conjugated with specific antibodies on their surface for continuous cell separation using ultrasonic standing wave. In acoustophoresis, cells being positive acoustic contrast particles migrate to pressure nodes. On the contrary we show that air-filled polymer-shelled microbubbles being strong negative acoustic contrast particles migrate to pressure antinodes at acoustic pressure amplitudes as low as 60 kPa. As a proof of principle, using the BAACS strategy, we demonstrate the separation of cancer cell line in a suspension with better than 75% efficiency. Moreover, 100% of the microbubble-cell conjugates migrated to the anti-node. Hence a better upstream affinity-capture has the potential to provide higher sorting efficiency. The BAACS technique may potentially provide a simplistic approach for similar sized selective isolation of cells, and is suited for applications in point of care.

Ort, förlag, år, upplaga, sidor
Springer, 2017. Vol. 19, nr 2, artikel-id 23
Nyckelord [en]
Cell sorting, acoustophoresis, microbubble, contrast agent, microfluidic separation
Nationell ämneskategori
Medicinsk bioteknologi
Identifikatorer
URN: urn:nbn:se:kth:diva-205293DOI: 10.1007/s10544-017-0157-4ISI: 000400547000005PubMedID: 28374278Scopus ID: 2-s2.0-85016958658OAI: oai:DiVA.org:kth-205293DiVA, id: diva2:1088338
Forskningsfinansiär
EU, FP7, Sjunde ramprogrammet, 115153Science for Life Laboratory - a national resource center for high-throughput molecular bioscience
Anmärkning

QC 20170515

Tillgänglig från: 2017-04-12 Skapad: 2017-04-12 Senast uppdaterad: 2019-01-30Bibliografiskt granskad

Open Access i DiVA

Fulltext saknas i DiVA

Övriga länkar

Förlagets fulltextPubMedScopus

Personposter BETA

Grishenkov, DmitryWiklund, Martin

Sök vidare i DiVA

Av författaren/redaktören
Faridi, Muhammad AsimRamachandraiah, HarishaIranmanesh, Ida SadatGrishenkov, DmitryWiklund, MartinRussom, Aman
Av organisationen
Proteomik och nanobioteknologiScience for Life Laboratory, SciLifeLabTillämpad fysikMedicinsk bildteknikBiomedicinsk fysik och röntgenfysik
I samma tidskrift
Biomedical microdevices (Print)
Medicinsk bioteknologi

Sök vidare utanför DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetricpoäng

doi
pubmed
urn-nbn
Totalt: 448 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf