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Influence of Glycosylation on Interfacial Properties of Recombinant Mucins: Adsorption, Surface Forces, and Friction
KTH, Skolan för kemivetenskap (CHE), Kemi, Yt- och korrosionsvetenskap.
KTH, Skolan för kemivetenskap (CHE), Kemi, Yt- och korrosionsvetenskap. SP Technical Research Institute of Sweden.
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2017 (Engelska)Ingår i: Langmuir, ISSN 0743-7463, E-ISSN 1520-5827, Vol. 33, nr 18, s. 4386-4395Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Interfacial properties of two brush-with-anchor mucins, C-P55 and C-PSLex, have been investigated at the aqueous solution/poly(methyl methacrylate) (PMMA) interface. Both are recombinant mucin-type fusion proteins, produced by fusing the glycosylated mucin part of P-selectin glycoprotein ligand-1 (PSLG-1) to the Fc part of a mouse immunoglobulin in two different cells. They are mainly expressed as dimers upon production. Analysis of the O-glycans shows that the C-PSLex mucin has the longer and more branched side chains, but C-P55 has slightly higher sialic acid content. The adsorption of the mucins to PMMA surfaces was studied by quartz crystal microbalance with dissipation. The sensed mass, including the adsorbed mucin and water trapped in the layer, was found to be similar for these two mucin layers. Atomic force microscopy with colloidal probe was employed to study surface and friction forces between mucin-coated PMMA surfaces. Purely repulsive forces of steric origin were observed between mucin layers on compression, whereas a small adhesion was detected between both mucin layers on decompression. This was attributed to chain entanglement. The friction force between C-PSLex-coated PMMA is lower than that between C-P55-coated PMMA. at low loads, but vice versa at high loads. We discuss our results in terms of the differences in the glycosylation composition of these two mucins.

Ort, förlag, år, upplaga, sidor
American Chemical Society (ACS), 2017. Vol. 33, nr 18, s. 4386-4395
Nationell ämneskategori
Metallurgi och metalliska material
Identifikatorer
URN: urn:nbn:se:kth:diva-208823DOI: 10.1021/acs.langmuir.7b00030ISI: 000401221600002PubMedID: 28431467Scopus ID: 2-s2.0-85019122835OAI: oai:DiVA.org:kth-208823DiVA, id: diva2:1109118
Forskningsfinansiär
VetenskapsrådetStiftelsen för internationalisering av högre utbildning och forskning (STINT)Vetenskapsrådet, 621-2013-5895 324-2004-4434Knut och Alice Wallenbergs StiftelseAFA Försäkring
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QC 20170613

Tillgänglig från: 2017-06-13 Skapad: 2017-06-13 Senast uppdaterad: 2017-06-13Bibliografiskt granskad

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