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Cellular Uptake of Plain and SPION-Modified Microbubbles for Potential Use in Molecular Imaging
KTH, Skolan för teknik och hälsa (STH).ORCID-id: 0000-0002-9604-0511
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2017 (Engelska)Ingår i: Cellular and Molecular Bioengineering, ISSN 1865-5025, E-ISSN 1865-5033, Vol. 10, nr 6, s. 537-548Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Both diagnostic ultrasound (US) and magnetic resonance imaging (MRI) accuracy can be improved by using contrast enhancement. For US gas-filled microbubbles (MBs) or silica nanoparticles (SiNPs), and for MRI superparamagnetic or paramagnetic agents, contribute to this. However, interactions of MBs with the vascular wall and cells are not fully known for all contrast media. We studied the in vitro interactions between three types of non-targeted air-filled MBs with a polyvinyl-alcohol shell and murine macrophages or endothelial cells. The three MB types were plain MBs and two types that were labelled (internally and externally) with superparamagnetic iron oxide nanoparticles (SPIONs) for US/MRI bimodality. Cells were incubated with MBs and imaged by microscopy to evaluate uptake and adhesion. Interactions were quantified and the MB internalization was confirmed by fluorescence quenching of non-internalized MBs. Macrophages internalized each MB type within different time frames: plain MBs 6 h, externally labelled MBs 25 min and internally labelled MBs 2 h. An average of 0.14 externally labelled MBs per cell were internalized after 30 min and 1.34 after 2 h; which was 113% more MBs than the number of internalized internally labelled MBs. The macrophages engulfed these three differently modified new MBs at various rate, whereas endothelial cells did not engulf MBs. Polyvinyl-alcohol MBs are not taken up by endothelial cells. The MB uptake by macrophages is promoted by SPION labelling, in particular external such, which may be important for macrophage targeting.

Ort, förlag, år, upplaga, sidor
SPRINGER , 2017. Vol. 10, nr 6, s. 537-548
Nyckelord [en]
Polyvinyl-alcohol, Macrophages, Endothelial cells, Interactions, In vitro
Nationell ämneskategori
Medicinsk bioteknologi
Identifikatorer
URN: urn:nbn:se:kth:diva-217726DOI: 10.1007/s12195-017-0504-9ISI: 000414198100003Scopus ID: 2-s2.0-85027153168OAI: oai:DiVA.org:kth-217726DiVA, id: diva2:1159383
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QC 20171122

Tillgänglig från: 2017-11-22 Skapad: 2017-11-22 Senast uppdaterad: 2017-11-22Bibliografiskt granskad

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Härmark, Johan

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