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Simple and rapid methods for purification and characterization of active coagulants from the seeds of Vigna unguiculata and Parkinsonia aculeata
KTH, Skolan för arkitektur och samhällsbyggnad (ABE), Mark- och vattenteknik.
KTH, Skolan för bioteknologi (BIO), Miljömikrobiologi.
KTH, Skolan för bioteknologi (BIO), Miljömikrobiologi.
2007 (Engelska)Ingår i: Environmental technology, ISSN 0959-3330, E-ISSN 1479-487X, Vol. 28, nr 6, s. 671-681Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

The coagulating properties of aqueous crude extracts and purified proteins of Vigna unguicilata and Parkinsomia aculeata seeds, which are traditional water coagulants in rural areas of Tanzania, were studied. The coagulation activity assays were done using one millilitre (ml) of kaolin water samples. Coagulating proteins were purified in two-step ion exchange chromatography. The properties of coagulant protein were compared with Moringa oleifera. Coagulating components eluted by 0.6 M NaCl in both coagulants are cationic proteins that have the molecular mass of about 6 kDa, which is very similar to that of M. oleifera. The proteins of V. unguiculata and P. aculeata eluted by 0.3 M NaCl also harbour coagulation activity but proteins eluted with 0.6 M NaCl have higher activity. The dosage for coagulation using purified proteins of both coagulants is about 5 to 10 times lower than that of crude seed extracts. The optimum floc settling time of water treated by crude seed extracts and purified proteins ranged between two and two and half hours. Coagulating proteins of both coagulants elated by 0.6 M NaCl are thermoresistant and retained coagulation activity of 87% to 92% after boiling for two hours at 80 degrees C and one hour at 95 degrees C. Thermotolerant proteins of V. unguiculata eluted by 0.6 M NaCl and P. aculeata have wider pH range of 5.5 to 8.5 for coagulation activity than those of M. oleifera proteins. The present investigation reveals the possibility of using purified natural coagulants for water treatment to produce safe drinking water.

Ort, förlag, år, upplaga, sidor
2007. Vol. 28, nr 6, s. 671-681
Nyckelord [en]
coagulation activity; local seeds; proteins; turbidity removal; extraction
Nationell ämneskategori
Vattenteknik
Identifikatorer
URN: urn:nbn:se:kth:diva-8565DOI: 10.1080/09593332808618827ISI: 000247827800008PubMedID: 17624107Scopus ID: 2-s2.0-34447125279OAI: oai:DiVA.org:kth-8565DiVA, id: diva2:13922
Anmärkning
QC 20100825Tillgänglig från: 2008-06-02 Skapad: 2008-06-02 Senast uppdaterad: 2020-03-09Bibliografiskt granskad
Ingår i avhandling
1. Water Supply in Tanzania and Performance of Local Plant Materials in Purification of Turbid Water
Öppna denna publikation i ny flik eller fönster >>Water Supply in Tanzania and Performance of Local Plant Materials in Purification of Turbid Water
2008 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

Water supply services in urban and rural areas of Tanzania were reviewed and specific studies were carried out on water supply and on purification of turbid water sources using locally available plant materials in rural villages of Singida Rural District. The review showed that large proportions of urban and rural populations in Tanzania face acute water supply problems mainly due to poor planning, implementation and management of water supply projects, including an inability to address social, technical, operation and maintenance and financial issues. Laboratory-scale experiments studied the effectiveness of crude seed extracts (CSEs) and purified proteins of Vigna unguiculata (VUP), Parkinsonia aculeata (PAP) and Voandzeia subterranea (VS) seeds, which are used traditionally for clarification of turbid water. The VUP and PAP were purified from CSEs using simple and straightforward two-step ion exchange chromatography. The coagulant proteins are thermoresistant and have a wide pH range for coagulation activity. Coagulation of turbid waters with CSEs, VUP and PAP produced low sludge volumes and removed turbidity along with other inorganic contaminants in line with Tanzania drinking water quality standards. The PAP also showed antimicrobial effect against river water bacteria. Citrus fruit juice (CF) enhanced the coagulation of turbid water by CSEs and inhibited bacterial growth, rendering it useful for disinfection of water prior to drinking in rural areas. It was concluded that natural coagulants should not be regarded as a panacea for rural water supply problems, but rather a tool in the development of sustainable water supply services in Tanzania.

Ort, förlag, år, upplaga, sidor
Stockholm: KTH, 2008. s. xii, 38
Serie
Trita-LWR. PHD, ISSN 1650-8602 ; 1042
Nyckelord
Antibacterial effect; Coagulant proteins; Vigna unguiculata; Parkinsonia aculeata; Ion exchange; Tanzania water supply
Nationell ämneskategori
Vattenteknik
Identifikatorer
urn:nbn:se:kth:diva-4781 (URN)978-91-7415-016-2 (ISBN)
Disputation
2008-06-10, F3, Conference Hall, Lindstedsvägen 26, KTH, Stockholm, Sweden, 10:15
Opponent
Handledare
Anmärkning
QC 20100825Tillgänglig från: 2008-06-02 Skapad: 2008-06-02 Senast uppdaterad: 2010-08-25Bibliografiskt granskad

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Marobhe, NancyDalhammar, GunnelKuttuva, Gunaratna R.
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