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Carbohydrate-active enzymes involved in the secondary cell wall biogenesis in hybrid aspen
KTH, Skolan för bioteknologi (BIO).ORCID-id: 0000-0002-8576-4370
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2005 (Engelska)Ingår i: Plant Physiology, ISSN 0032-0889, E-ISSN 1532-2548, Vol. 137, nr 3, s. 983-997Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Wood formation is a fundamental biological process with significant economic interest. While lignin biosynthesis is currently relatively well understood, the pathways leading to the synthesis of the key structural carbohydrates in wood fibers remain obscure. We have used a functional genomics approach to identify enzymes involved in carbohydrate biosynthesis and remodeling during xylem development in the hybrid aspen Populus tremula x tremuloides. Microarrays containing cDNA clones from different tissue-specific libraries were hybridized with probes obtained from narrow tissue sections prepared by cryosectioning of the developing xylem. Bioinformatic analyses using the sensitive tools developed for carbohydrate-active enzymes allowed the identification of 25 xylem-specific glycosyltransferases belonging to the Carbohydrate-Active EnZYme families GT2, GT8, GT14, GT31, GT43, GT47, and GT61 and nine glycosidases (or transglycosidases) belonging to the Carbohydrate-Active EnZYme families GH9, GH10, GH16, GH17, GH19, GH28, GH35, and GH51. While no genes encoding either polysaccharide lyases or carbohydrate esterases were found among the secondary wall-specific genes, one putative O-acetyltransferase was identified. These wood-specific enzyme genes constitute a valuable resource for future development of engineered fibers with improved performance in different applications.

Ort, förlag, år, upplaga, sidor
2005. Vol. 137, nr 3, s. 983-997
Nyckelord [en]
arabidopsis-thaliana, cellulose synthesis, gene-expression, mesophyll-cells, xyloglucan galactosyltransferase, functional genomics, beta-galactosidase, tracheary elements, catalytic subunit, molecular-cloning
Nationell ämneskategori
Växtbioteknologi
Identifikatorer
URN: urn:nbn:se:kth:diva-14637DOI: 10.1104/pp.104.055087ISI: 000227957200021PubMedID: 15734915Scopus ID: 2-s2.0-20444437284OAI: oai:DiVA.org:kth-14637DiVA, id: diva2:332678
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QC 20100525

Tillgänglig från: 2010-08-05 Skapad: 2010-08-05 Senast uppdaterad: 2017-12-12Bibliografiskt granskad

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Aspeborg, HenrikSterky, Fredrik

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Aspeborg, HenrikNilsson, PeterSterky, FredrikTeeri, Tuula T.
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Skolan för bioteknologi (BIO)Proteomik (stängd 20130101)Glykovetenskap
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