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Selection of affibody molecules to the ligand-binding site of the insulin-like growth factor-1 receptor
KTH, Skolan för bioteknologi (BIO), Molekylär Bioteknologi (stängd 20130101).
KTH, Skolan för bioteknologi (BIO), Molekylär Bioteknologi (stängd 20130101).ORCID-id: 0000-0001-7034-0850
KTH, Skolan för bioteknologi (BIO), Molekylär Bioteknologi (stängd 20130101).
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2010 (Engelska)Ingår i: Biotechnology and applied biochemistry, ISSN 0885-4513, E-ISSN 1470-8744, Vol. 55, s. 99-109Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Affibody molecules binding to the site of hormone interaction in IGF-IR (insulin-like growth factor-I receptor) were successfully selected by phage-display technology employing a competitive-elution strategy during biopanning, whereby release of receptor-bound phagemids was accomplished by competition with IGFI (insulin-like growth factor-I). In non-competitive selections, the elution of receptor-bound phagemids was performed by imidazole or low-pH incubation, which also resulted in the isolation of affibody molecules that could bind to the receptor. An ELISA-based assay showed that the affibody molecules generated by IGF-I competition during elution, in addition to affibody molecules generated in the noncompetitive selections, could compete with IGF-I for binding to the receptor. The affinities of the isolated variants to IGF-IR-overexpressing MCF-7 cells were determined and ranged from high nanomolar to 2.3 nM. The most promising variant, Z(4;40), was shown to recognize IGF- IR efficiently in several different contexts: in analyses based on flow cytometry, fluorescence microscopy and receptor pull-down from cell extracts. In addition, when Z, was added to the medium of MCF-7 cells that were dependent on IGF-I for efficient growth, it was found to have a dose-dependent growth-inhibitory effect on the cells. Applications of affibody-based reagents for quantitative and qualitative analyses of IGF- I R status, as well as applications of affibody-based reagents for therapy, are discussed.

Ort, förlag, år, upplaga, sidor
2010. Vol. 55, s. 99-109
Nyckelord [en]
affibody molecule, competitive elution, insulin-like growth factor-1, receptor (IGF-IR), phage display, human-breast-cancer, monoclonal-antibody, carcinoma cells, proteins, domain, expression, libraries, scaffold
Nationell ämneskategori
Industriell bioteknik
Identifikatorer
URN: urn:nbn:se:kth:diva-19387DOI: 10.1042/ba20090226ISI: 000276539400005Scopus ID: 2-s2.0-77954828431OAI: oai:DiVA.org:kth-19387DiVA, id: diva2:337434
Forskningsfinansiär
Vetenskapsrådet, 621-20055134
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QC 20100525

Tillgänglig från: 2010-08-05 Skapad: 2010-08-05 Senast uppdaterad: 2017-12-12Bibliografiskt granskad

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Lundberg, EmmaGräslund, Torbjörn

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Li, JingjingLundberg, EmmaVernet, ErikGräslund, Torbjörn
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Biotechnology and applied biochemistry
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