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Self-assembled and self-sorted array of chemically active beads for analytical and biochemical screening
KTH, Tidigare Institutioner                               , Bioteknologi.
KTH, Tidigare Institutioner                               , Kemi.ORCID-id: 0000-0002-1743-7650
KTH, Tidigare Institutioner                               , Signaler, sensorer och system.ORCID-id: 0000-0001-9552-4234
2002 (Engelska)Ingår i: Talanta: The International Journal of Pure and Applied Analytical Chemistry, ISSN 0039-9140, E-ISSN 1873-3573, Vol. 56, nr 2, s. 301-308Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

A technique for generating a general screening platform consisting of dots of immobilized beads on silicon has been developed via self-sorting and -assembly of different kinds of beads. The dots are defined by a teflon-like film, which due to its hydrophobic characteristics also prevents cross-contamination of liquid from different dots. To enable functionalization of individual dots with different target molecules simultaneously a new way of microcontact printing has been explored where different target solutions are printed in parallel using one stamp. In order to show that this platform can be designed for both biochemical assays and organic chemistry, streptavidin-, amino- and hydroxy-functionalized beads have been self-sorted and -assembled both on separate and common platforms. The self-sorting and -arrangement are based on surface chemistry only, which has not previously been reported. Beads of different sizes and material have successfully been immobilized in line patterns as narrow as 5 mum. Besides silicon, quartz and polyethylene have also been used as substrates.

Ort, förlag, år, upplaga, sidor
2002. Vol. 56, nr 2, s. 301-308
Nyckelord [en]
microcontact printing, self-sorting, self-assembly, beads, monolayer, miniaturization, monolayers, proteins
Identifikatorer
URN: urn:nbn:se:kth:diva-21336ISI: 000174005400006OAI: oai:DiVA.org:kth-21336DiVA, id: diva2:340034
Anmärkning
QC 20100525Tillgänglig från: 2010-08-10 Skapad: 2010-08-10 Senast uppdaterad: 2017-12-12Bibliografiskt granskad

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Moberg, ChristinaStemme, Göran

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Andersson, HeleneMoberg, ChristinaStemme, Göran
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BioteknologiKemiSignaler, sensorer och system
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Talanta: The International Journal of Pure and Applied Analytical Chemistry

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