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Engineered Pyranose 2-Oxidase: Efficiently Turning Sugars into Electrical Energy
KTH, Skolan för bioteknologi (BIO), Glykovetenskap.
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2010 (Engelska)Ingår i: Electroanalysis, ISSN 1040-0397, E-ISSN 1521-4109, Vol. 22, nr 7-8, s. 813-820Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Due to the recent interest in enzymatic biofuel cells (BECs), sugar oxidizing enzymes other than the commonly used glucose oxidase are gaining more importance as possible bioelements of implantable microscale-devices, which can, for example, be used in biosensors and pacemakers. In this study we used rational and semi-rational protein design to improve the catalytic activity of the enzyme pyranose 2-oxidase (P2Ox) with its alternative soluble electron acceptors 1,4-benzoquinone and ferricenium ion, which can serve as electron mediators, to possibly boost the power output of enzymatic BECs. Using a screening assay based on 96-well plates, we identified the variant H450G, which showed lower K-M and higher k(cat) values for both 1,4-benzoquinone and ferricenium ion compared to the wild-type enzyme, when either D-glucose or D-galactose were used as saturating electron donors. Besides this variant, we analyzed the variants V546C and T169G/V546C for their possible application in enzymatic BECs. The results obtained in homogeneous solution were compared with those obtained when P2Ox was immobilized on the surface of graphite electrodes and either "wired" to an osmium redox polymer or using soluble 1,4-benzoquinone as mediator. According to the spectrophotometrically determined kinetic constants, the possible energy output, measured in flow injection analysis experiments with these variants, increased up to 4-fold compared to systems employing the wild-type enzyme. Our results show that by increasing the catalytic activity of the redox enzyme P2Ox with its alternative electron acceptors 1,4-benzoquinone and ferricenium ion, it is possible to achieve a higher energy output of an enzymatic BFC when using the same concentration of sugar substrate.

Ort, förlag, år, upplaga, sidor
2010. Vol. 22, nr 7-8, s. 813-820
Nyckelord [en]
Pyranose 2-oxidase, Sugar oxidoreductases, Rational protein design, Enzymatic biofuel cell, Mediated electron transfer, Osmium redox polymer, 1, 4-Benzoquinone, Fuel cells
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Analytisk kemi
Identifikatorer
URN: urn:nbn:se:kth:diva-28232DOI: 10.1002/elan.200980015ISI: 000277255400012Scopus ID: 2-s2.0-77950996000OAI: oai:DiVA.org:kth-28232DiVA, id: diva2:389432
Forskningsfinansiär
Vetenskapsrådet, 621-2007-4124
Anmärkning
QC 20110119Tillgänglig från: 2011-01-19 Skapad: 2011-01-12 Senast uppdaterad: 2017-12-11Bibliografiskt granskad

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