kth.sePublikationer
Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Isothermal solid-phase recombinase polymerase amplification on microfluidic digital versatile discs (DVDs)
KTH, Skolan för bioteknologi (BIO), Proteomik och nanobioteknologi.
KTH, Skolan för bioteknologi (BIO), Proteomik och nanobioteknologi.ORCID-id: 0000-0002-0242-358X
Visa övriga samt affilieringar
2015 (Engelska)Ingår i: RSC Advances, E-ISSN 2046-2069, Vol. 5, nr 38, s. 29987-29995Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

A new advancement in massive DNA-based screening in limited-resource settings is demonstrated through the incorporation of easy-to-fabricate microfluidic chambers on digital versatile discs (DVDs) to perform isothermal recombinase polymerase amplification (RPA) in a microarray format. Standard un-modified DVD discs and commercial drives are used for the low-cost detection method. DNA primers were printed in a microarray format on the polycarbonate surfaces of DVDs with integrated control spots to guarantee the absence of false-negatives and false-positives. The solid-phase amplification assay, including the washing protocols and development reaction, was performed by the dispensation of solutions through the inlet and by controlling the flow-movement by DVD drive centrifugation. The final disc with reaction products was inserted into a DVD player and microarray images were captured and automatically processed. This simple approach was applied for the screening of genetically modified organisms (GMOs) in food samples. The limit of detection was 7 mu g g(-1), which is well below the EU regulation limit for GMOs in food products. Therefore, the only required materials for food safety monitoring were standard store-bought DVDs, plastic chambers, tips, pipettes, an oven, and a standard DVD drive. The proposed strategy allows an integrated microarray system with low manipulation, reduced sample volume, and portability, which are beneficial for low-resource settings.

Ort, förlag, år, upplaga, sidor
2015. Vol. 5, nr 38, s. 29987-29995
Nationell ämneskategori
Nanoteknik
Identifikatorer
URN: urn:nbn:se:kth:diva-171737DOI: 10.1039/c5ra02778kISI: 000352789500047Scopus ID: 2-s2.0-84928910817OAI: oai:DiVA.org:kth-171737DiVA, id: diva2:844681
Forskningsfinansiär
EU, FP7, Sjunde ramprogrammet, PAID-00-12 (UPV, GVA)
Anmärkning

QC 20150807

Tillgänglig från: 2015-08-07 Skapad: 2015-08-05 Senast uppdaterad: 2024-03-18Bibliografiskt granskad

Open Access i DiVA

Fulltext saknas i DiVA

Övriga länkar

Förlagets fulltextScopus

Person

Russom, Aman

Sök vidare i DiVA

Av författaren/redaktören
Amasia, MaryRussom, Aman
Av organisationen
Proteomik och nanobioteknologi
I samma tidskrift
RSC Advances
Nanoteknik

Sök vidare utanför DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetricpoäng

doi
urn-nbn
Totalt: 72 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf