Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Self-assembled magnetic bead chains for sensitivity enhancement of microfluidic electrochemical biosensor platforms
KTH, Skolan för bioteknologi (BIO), Proteinteknologi. University of Freiburg, Germany.
Visa övriga samt affilieringar
2015 (Engelska)Ingår i: Lab on a Chip, ISSN 1473-0197, E-ISSN 1473-0189, Vol. 15, nr 22, s. 4314-4321Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

In this paper, we present a novel approach to enhance the sensitivity of microfluidic biosensor platforms with self-assembled magnetic bead chains. An adjustable, more than 5-fold sensitivity enhancement is achieved by introducing a magnetic field gradient along a microfluidic channel by means of a soft-magnetic lattice with a 350 mu m spacing. The alternating magnetic field induces the self-assembly of the magnetic beads in chains or clusters and thus improves the perfusion and active contact between the analyte and the beads. The soft-magnetic lattices can be applied independent of the channel geometry or chip material to any microfluidic biosensing platform. At the same time, the bead-based approach achieves chip reusability and shortened measurement times. The bead chain properties and the maximum flow velocity for bead retention were validated by optical microscopy in a glass capillary. The magnetic actuation system was successfully validated with a biotin-streptavidin model assay on a low-cost electrochemical microfluidic chip, fabricated by dry-film photoresist technology (DFR). Labelling with glucose oxidase (GOx) permits rapid electrochemical detection of enzymatically produced H2O2.

Ort, förlag, år, upplaga, sidor
2015. Vol. 15, nr 22, s. 4314-4321
Nationell ämneskategori
Biokemi och molekylärbiologi Nanoteknik
Identifikatorer
URN: urn:nbn:se:kth:diva-177980DOI: 10.1039/c5lc00796hISI: 000364072300008PubMedID: 26394820Scopus ID: 2-s2.0-84946092223OAI: oai:DiVA.org:kth-177980DiVA, id: diva2:875916
Anmärkning

QC 20151202

Tillgänglig från: 2015-12-02 Skapad: 2015-11-30 Senast uppdaterad: 2017-12-01Bibliografiskt granskad

Open Access i DiVA

Fulltext saknas i DiVA

Övriga länkar

Förlagets fulltextPubMedScopus

Sök vidare i DiVA

Av författaren/redaktören
Horak, Josef
Av organisationen
Proteinteknologi
I samma tidskrift
Lab on a Chip
Biokemi och molekylärbiologiNanoteknik

Sök vidare utanför DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetricpoäng

doi
pubmed
urn-nbn
Totalt: 347 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf