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Sub 30-nm resolution compact x-ray microscopy
KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Biomedicinsk fysik och röntgenfysik.
KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Biomedicinsk fysik och röntgenfysik.
KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Biomedicinsk fysik och röntgenfysik.
KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Biomedicinsk fysik och röntgenfysik.ORCID-id: 0000-0003-2745-6289
Vise andre og tillknytning
2006 (engelsk)Inngår i: Journal of Microscopy, ISSN 0022-2720, E-ISSN 1365-2818Artikkel i tidsskrift (Annet vitenskapelig) Submitted
sted, utgiver, år, opplag, sider
2006.
HSV kategori
Identifikatorer
URN: urn:nbn:se:kth:diva-6203OAI: oai:DiVA.org:kth-6203DiVA, id: diva2:10847
Merknad
QS 20120316Tilgjengelig fra: 2006-10-03 Laget: 2006-10-03 Sist oppdatert: 2020-01-30bibliografisk kontrollert
Inngår i avhandling
1. Compact Soft X-Ray Microscopy: Image Processing and Instrumentation
Åpne denne publikasjonen i ny fane eller vindu >>Compact Soft X-Ray Microscopy: Image Processing and Instrumentation
2006 (engelsk)Doktoravhandling, med artikler (Annet vitenskapelig)
Abstract [en]

Soft x-ray microscopy is a powerful technique for natural-contrast, high-resolution imaging of organic materials. This Thesis describes new instrumentational and new image-processing methods to improve the image quality of the compact x-ray microscope at the Biomedical & X-Ray Physics division at KTH. The microscope is based on a laser-plasma source combined with different condenser optics, either multilayer mirrors or zone plates. Imaging is performed by micro zone plates. The microscope works in the water window (\lambda = 2.3-4.4 nm), where the attenuation lengths of oxygen and carbon differ strongly, providing high natural contrast for carbon-containing specimens in an aqueous environment.

By optimizing the properties of the laser-plasma source and fabricating multilayer mirrors with high, uniform reflectivity, the performance of the microscope's illumination system could be improved and exposure times decreased significantly to about 2 min for imaging dry samples and 5 min for imaging wet samples. For imaging of wet samples, a wet-specimen chamber was developed, which is vacuum-compatible. Since it is horizontally mounted in the microscope, it offers advantages for investigations in polymer and soil science.

To improve the quality of images taken by the compact x-ray microscope an image-restoration algorithm was developed. Denoising is performed by a filtering algorithm based on the discrete wavelet transform. This algorithm shows advantages compared to Fourier-based algorithms, since the filtering of spatial frequencies is done locally. An improvement in exposure time by a factor of about 2 could be realized without loss of image information.

To stimulate experiments on functional imaging in x-ray microscopy an image-analysis algorithm for identifying colloidal-gold particles was developed. This algorithm is based on a combination of a threshold with respect to the local absorption and a shape discrimination, realized by fitting a Gaussian profile to the potential particles. The algorithm was evaluated and optimized on images taken by the transmission x-ray microscope at BESSY II. The size-selective identification and localization of single gold particles down to a diameter of 50 nm was demonstrated.

sted, utgiver, år, opplag, sider
Stockholm: KTH, 2006. s. viii, 51
Serie
Trita-FYS, ISSN 0280-316X ; 2006:60
Emneord
X-ray microscopy
HSV kategori
Identifikatorer
urn:nbn:se:kth:diva-4128 (URN)91-7178-458-6 (ISBN)
Disputas
2006-10-20, FD 5, Albanova, 10:30
Opponent
Veileder
Merknad
QC 20100920Tilgjengelig fra: 2006-10-03 Laget: 2006-10-03 Sist oppdatert: 2010-09-20bibliografisk kontrollert

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Holmberg, AndersHertz, Hans M.

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Takman, Per A. C.Stollberg, HeideJohansson, Göran A.Holmberg, AndersLindblom, MagnusHertz, Hans M.
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