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Experimental strategies for imaging bioparticles with femtosecond hard X-ray pulses
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2017 (engelsk)Inngår i: IUCrJ, ISSN 0972-6918, E-ISSN 2052-2525, Vol. 4, s. 251-262Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

This study explores the capabilities of the Coherent X-ray Imaging Instrument at the Linac Coherent Light Source to image small biological samples. The weak signal from small samples puts a significant demand on the experiment. Aerosolized Omono River virus particles of similar to 40 nm in diameter were injected into the submicrometre X-ray focus at a reduced pressure. Diffraction patterns were recorded on two area detectors. The statistical nature of the measurements from many individual particles provided information about the intensity profile of the X-ray beam, phase variations in the wavefront and the size distribution of the injected particles. The results point to a wider than expected size distribution (from similar to 35 to similar to 300 nm in diameter). This is likely to be owing to nonvolatile contaminants from larger droplets during aerosolization and droplet evaporation. The results suggest that the concentration of nonvolatile contaminants and the ratio between the volumes of the initial droplet and the sample particles is critical in such studies. The maximum beam intensity in the focus was found to be 1.9 * 10(12) photons per mu m(2) per pulse. The full-width of the focus at half-maximum was estimated to be 500 nm (assuming 20% beamline transmission), and this width is larger than expected. Under these conditions, the diffraction signal from a sample-sized particle remained above the average background to a resolution of 4.25 nm. The results suggest that reducing the size of the initial droplets during aerosolization is necessary to bring small particles into the scope of detailed structural studies with X-ray lasers.

sted, utgiver, år, opplag, sider
INT UNION CRYSTALLOGRAPHY , 2017. Vol. 4, s. 251-262
Emneord [en]
X-ray diffraction, free-electron laser, flash X-ray imaging, diffraction before destruction, virus, Omono River virus, OmRV
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Identifikatorer
URN: urn:nbn:se:kth:diva-207895DOI: 10.1107/S2052252517003591ISI: 000400460500008Scopus ID: 2-s2.0-85018306872OAI: oai:DiVA.org:kth-207895DiVA, id: diva2:1103694
Merknad

Correction in DOI: 10.1107/S2052252519004317

QC 20170530

Tilgjengelig fra: 2017-05-30 Laget: 2017-05-30 Sist oppdatert: 2019-07-15bibliografisk kontrollert

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