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Study of Real-time Lectin–Carbohydrate Interactions on the Surface of a Quartz Crystal Microbalance
KTH, Skolan för kemivetenskap (CHE), Kemi.
KTH, Skolan för kemivetenskap (CHE), Kemi.ORCID-id: 0000-0002-1533-6514
2005 (Engelska)Ingår i: Biosensors & bioelectronics, ISSN 0956-5663, E-ISSN 1873-4235, Vol. 21, nr 1, s. 60-66Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

A quartz crystal microbalance (QCM) biosensor system for lectin-carbohydrate interactions has been developed. Yeast mannan was immobilised on polystyrene-coated quartz crystals, and interactions tested with the lectin concanavalin A (Con A). The biosensor could be easily operated, where mannan immobilisation and all binding analyses were performed in real-time using a flow-through system. The apparent binding constant for yeast mannan to Con A was estimated to be 0.4 μM, well in accordance to reported literature values. In addition, the effective concentration values (EC50-values) for a series of mannose/mannoside ligands, acting as competitors to the mannan/Con A interaction, were determined to range from 0.18 to 5.3 mM, in good correlation with a related enzyme-labelled lectin assay (ELLA) protocol

Ort, förlag, år, upplaga, sidor
2005. Vol. 21, nr 1, s. 60-66
Nyckelord [en]
Carbohydrates, Glycochemistry, Lectins; Molecular recognition, Quartz crystal microbalance
Nationell ämneskategori
Organisk kemi
Identifikatorer
URN: urn:nbn:se:kth:diva-6389DOI: 10.1016/j.bios.2004.10.006ISI: 000232268900006PubMedID: 15967351Scopus ID: 2-s2.0-20444439074OAI: oai:DiVA.org:kth-6389DiVA, id: diva2:11085
Anmärkning
QC 20100915Tillgänglig från: 2006-11-22 Skapad: 2006-11-22 Senast uppdaterad: 2020-03-09Bibliografiskt granskad
Ingår i avhandling
1. Carbohydrate Synthesis and Study of Carbohydrate-Lectin Interactions Using QCM Biosensors and Microarray Technologies
Öppna denna publikation i ny flik eller fönster >>Carbohydrate Synthesis and Study of Carbohydrate-Lectin Interactions Using QCM Biosensors and Microarray Technologies
2006 (Engelska)Doktorsavhandling, sammanläggning (Övrigt vetenskapligt)
Abstract [en]

Interactions between carbohydrates and proteins are increasingly being recognized as crucial in many biological processes, such as cellular adhesion and communication. In order to investigate the interactions of carbohydrates and proteins, the development of efficient analytic technologies, as well as novel strategies for the synthesis of carbohydrates, have to be explored. To date, several methods have been exploited to analyze interactions of carbohydrates and proteins, for example, biosensors, nuclear magnetic resonance (NMR); enzyme-linked immunosorbent assays (ELISA), X-ray crystallography and array technologies. This thesis describes the development of novel strategies for the synthesis of carbohydrates, as well as new efficient strategies to Quartz Crystal Microbalance- (QCM-) biosensors and carbohydrate microarrays technologies. These methodologies have been used to probe carbohydrate-lectin-interactions for a range of plant and animal lectins.

Ort, förlag, år, upplaga, sidor
Stockholm: KTH, 2006. s. 106
Serie
Trita-IOK, ISSN 1100-7974 ; 2006:106
Nyckelord
Lectins; Carbohydrates; Molecular recognition; Biosensors; Quartz crystal microbalance; Carbohydrate microarrays.
Nationell ämneskategori
Organisk kemi
Identifikatorer
urn:nbn:se:kth:diva-4177 (URN)91-7178-503-5 (ISBN)
Disputation
2006-12-01, D3, Lindstedtsvägen 5, Stockholm, 10:00
Opponent
Handledare
Anmärkning
QC 20100915Tillgänglig från: 2006-11-22 Skapad: 2006-11-22 Senast uppdaterad: 2019-12-20Bibliografiskt granskad

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Förlagets fulltextPubMedScopushttp://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TFC-4DVBJ9B-2&_user=4478132&_coverDate=07%2F15%2F2005&_alid=493485618&_rdoc=1&_fmt=summary&_orig=search&_cdi=5223&_sort=d&_docanchor=&view=c&_acct=C000034958&_version=1&_urlVersion=0&_userid=4478132&md5=3949377cbdf6f7b3676561e935fc818d

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