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Photogenerated Carbohydrate Microarrays
KTH, Skolan för kemivetenskap (CHE), Kemi.
KTH, Skolan för kemivetenskap (CHE), Kemi.
KTH, Skolan för kemivetenskap (CHE), Kemi.
KTH, Skolan för bioteknologi (BIO).
Vise andre og tillknytning
2007 (engelsk)Inngår i: ChemBioChem (Print), ISSN 1439-4227, E-ISSN 1439-7633, Vol. 8, nr 2, s. 166-168Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Chemical Equation Presented) Sugars in a row. A new strategy for carbohydrate microarrays based on photochemical ligation of perfluorophenylazide-derivatized carbohydrates to PEO surfaces is presented. It constitutes a controllable and robust method of array fabrication, on the carbohydrate chemistry and on the surface-chemistry levels, and the resulting carbohydrate arrays can be efficiently used to reveal the recognition patterns of carbohydrate-binding proteins.

sted, utgiver, år, opplag, sider
Wiley-VCH Verlagsgesellschaft, 2007. Vol. 8, nr 2, s. 166-168
Emneord [en]
carbohydrates, lectins, microarrays, photochemistry, surfaces
HSV kategori
Identifikatorer
URN: urn:nbn:se:kth:diva-6392DOI: 10.1002/cbic.200600447ISI: 000243810000003PubMedID: 17154195Scopus ID: 2-s2.0-33846914211OAI: oai:DiVA.org:kth-6392DiVA, id: diva2:11088
Merknad

QC 20100915

Tilgjengelig fra: 2006-11-22 Laget: 2006-11-22 Sist oppdatert: 2019-12-20bibliografisk kontrollert
Inngår i avhandling
1. Carbohydrate Synthesis and Study of Carbohydrate-Lectin Interactions Using QCM Biosensors and Microarray Technologies
Åpne denne publikasjonen i ny fane eller vindu >>Carbohydrate Synthesis and Study of Carbohydrate-Lectin Interactions Using QCM Biosensors and Microarray Technologies
2006 (engelsk)Doktoravhandling, med artikler (Annet vitenskapelig)
Abstract [en]

Interactions between carbohydrates and proteins are increasingly being recognized as crucial in many biological processes, such as cellular adhesion and communication. In order to investigate the interactions of carbohydrates and proteins, the development of efficient analytic technologies, as well as novel strategies for the synthesis of carbohydrates, have to be explored. To date, several methods have been exploited to analyze interactions of carbohydrates and proteins, for example, biosensors, nuclear magnetic resonance (NMR); enzyme-linked immunosorbent assays (ELISA), X-ray crystallography and array technologies. This thesis describes the development of novel strategies for the synthesis of carbohydrates, as well as new efficient strategies to Quartz Crystal Microbalance- (QCM-) biosensors and carbohydrate microarrays technologies. These methodologies have been used to probe carbohydrate-lectin-interactions for a range of plant and animal lectins.

sted, utgiver, år, opplag, sider
Stockholm: KTH, 2006. s. 106
Serie
Trita-IOK, ISSN 1100-7974 ; 2006:106
Emneord
Lectins; Carbohydrates; Molecular recognition; Biosensors; Quartz crystal microbalance; Carbohydrate microarrays.
HSV kategori
Identifikatorer
urn:nbn:se:kth:diva-4177 (URN)91-7178-503-5 (ISBN)
Disputas
2006-12-01, D3, Lindstedtsvägen 5, Stockholm, 10:00
Opponent
Veileder
Merknad
QC 20100915Tilgjengelig fra: 2006-11-22 Laget: 2006-11-22 Sist oppdatert: 2019-12-20bibliografisk kontrollert

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