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Slipdisc: A versatile sample preparation platform for point of care diagnostics
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology.
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology.
KTH, School of Biotechnology (BIO), Proteomics and Nanobiotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0001-5199-0663
KTH, School of Chemical Science and Engineering (CHE), Fibre and Polymer Technology, Fibre Technology.ORCID iD: 0000-0003-1874-2187
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2017 (English)In: RSC Advances, ISSN 2046-2069, E-ISSN 2046-2069, Vol. 7, no 56, p. 35048-35054Article in journal (Refereed) Published
Abstract [en]

We report a microfluidic sample preparation platform called "Slipdisc" based on slipchip technology. Slipdisc is a rotational slipchip that uses a unique hand-wound clockwork mechanism for precise movement of specially fabricated polycarbonate discs. In operation, the microchannels and microchambers carved on the closely aligned microfluidic discs convert from continuous filled paths to defined compartments using the slip movement. The clockwork mechanism introduced here is characterised by a food dye experiment and a conventional HRP TMB reaction before measuring lactate dehydrogenase (LDH) enzyme levels, which is a crucial biomarker for neonatal diagnostics. The colorimetry based detection of LDH was performed with an unmodified camera and an image analysis procedure based on normalising images and observing changes in red channel intensity. The analysis showed a close to unity coefficient of determination (R2 = 0.96) in detecting the LDH concentration when compared with a standard Chemical Analyser, demonstrating the excellent performance of the slipdisc platform with colorimetric detection. The versatile point of care sample preparation platform should ideally be suited for a multitude of applications at resource-limited settings.

Place, publisher, year, edition, pages
Royal Society of Chemistry , 2017. Vol. 7, no 56, p. 35048-35054
Keywords [en]
Chemical analysis, Chemical detection, Clocks, Colorimetric analysis, Colorimetry, Coefficient of determination, Colorimetric detection, Lactate dehydrogenase, Micro-chambers, Point of care, Point of care diagnostic, Red channels, Sample preparation, Microfluidics
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:kth:diva-219077DOI: 10.1039/c7ra05209jISI: 000405811400015Scopus ID: 2-s2.0-85025080747OAI: oai:DiVA.org:kth-219077DiVA, id: diva2:1161966
Note

QC 20171201

Available from: 2017-12-01 Created: 2017-12-01 Last updated: 2019-02-28Bibliographically approved
In thesis
1. Point of care microfluidic tool development for resource limited settings
Open this publication in new window or tab >>Point of care microfluidic tool development for resource limited settings
2019 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The development of point of care diagnostics using recent advances in microfluidics have the potential to transform health care in several ways, especially in resource limited settings with limited access to advanced health care infrastructure. However, translating a point of care device to reality is often a challenging task because of the complexities involved in integrating a number of diverse engineering concepts into an easy to use, accurate and portable device. This thesis focuses on miniaturization of crucial diagnostic laboratory tools, that can be used in a portable point of care format without compromising on the accuracy or performance. The first part of the thesis (Paper I-III) focuses on understanding and applying elasto-inertial microfluidics, which is a label-free and passive bio-particle sorting and separation method. A basic understanding of particle trajectories in both inertial (Paper I) and visco-elastic flows (Paper II) is established, followed by an investigation on the combined effects of inertia and elasticity (Paper III). The second part of the thesis (Paper IV-VI) focuses on developing integrated microfluidic platforms, each of which addresses different aspects of point of care diagnostic applications. The applications include neonatal diagnostics using a hand-driven Slipdisc technique (Paper IV), rapid nucleic acid quantification using a novel precipitate-based detection on a centrifugal microfluidics platform (Paper V), and hematocrit level measurement in blood using a portable lab-on- Disc platform operated by a mobile phone (Paper VI). The proof of concept microfluidic tools presented in the scope of this thesis have the potential to replace a number of functions of standard laboratory equipment, at a fraction of the price and without compromising performance. Hence, the different methods developed should contribute towards decentralization of medical testing laboratories, making healthcare accessible to one and all.

Place, publisher, year, edition, pages
Stockholm: KTH Royal Institute of Technology, 2019. p. 63
Series
TRITA-CBH-FOU ; 2019:15
Keywords
Blood, control, cell separation, centrifugal microfluidics, diagnostics, elasto-inertial, hematocrit level, microfluidics, neonatal diagnostics, nucleic acid quantification, point of care, particle focusing, resource limited settings.
National Category
Medical and Health Sciences Engineering and Technology
Research subject
Biotechnology
Identifiers
urn:nbn:se:kth:diva-244825 (URN)978-91-7873-122-0 (ISBN)
Public defence
2019-03-29, Air & Fire auditorium, Science for Life Laboratory, Tomtebodavägen 23, Solna, 10:00 (English)
Opponent
Supervisors
Note

QC 20190228

Available from: 2019-02-28 Created: 2019-02-28 Last updated: 2019-02-28Bibliographically approved

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Banerjee, IndradumnaSalih, TagridRamachandraiah, HarishaErlandsson, JohanPettersson, TorbjörnRussom, Aman

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