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The function of two type II metacaspases in woody tissues of Populus trees
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2018 (English)In: New Phytologist, ISSN 0028-646X, E-ISSN 1469-8137, Vol. 217, no 4, p. 1551-1565Article in journal (Refereed) Published
Abstract [en]

Metacaspases (MCs) are cysteine proteases that are implicated in programmed cell death of plants. AtMC9 (Arabidopsis thaliana Metacaspase9) is a member of the Arabidopsis MC family that controls the rapid autolysis of the xylem vessel elements, but its downstream targets in xylem remain uncharacterized. PttMC13 and PttMC14 were identified as AtMC9 homologs in hybrid aspen (Populustremulaxtremuloides). A proteomic analysis was conducted in xylem tissues of transgenic hybrid aspen trees which carried either an overexpression or an RNA interference construct for PttMC13 and PttMC14. The proteomic analysis revealed modulation of levels of both previously known targets of metacaspases, such as Tudor staphylococcal nuclease, heat shock proteins and 14-3-3 proteins, as well as novel proteins, such as homologs of the PUTATIVE ASPARTIC PROTEASE3 (PASPA3) and the cysteine protease RD21 by PttMC13 and PttMC14. We identified here the pathways and processes that are modulated by PttMC13 and PttMC14 in xylem tissues. In particular, the results indicate involvement of PttMC13 and/or PttMC14 in downstream proteolytic processes and cell death of xylem elements. This work provides a valuable reference dataset on xylem-specific metacaspase functions for future functional and biochemical analyses.

Place, publisher, year, edition, pages
Wiley , 2018. Vol. 217, no 4, p. 1551-1565
Keywords [en]
aspartic protease, cellular autolysis, cysteine protease, metacaspase, Populus, programmed cell death, wood formation, xylem differentiation
National Category
Plant Biotechnology
Identifiers
URN: urn:nbn:se:kth:diva-223251DOI: 10.1111/nph.14945ISI: 000424284400017PubMedID: 29243818Scopus ID: 2-s2.0-85037999750OAI: oai:DiVA.org:kth-223251DiVA, id: diva2:1183744
Funder
Swedish Energy Agency, 2010-000431VINNOVA, 2015-02290Swedish Research Council, 621-2013-4949The Kempe Foundations, SMK-1340Swedish Research Council Formas, 232-2009-1698
Note

QC 20180219

Available from: 2018-02-19 Created: 2018-02-19 Last updated: 2018-02-19Bibliographically approved

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Bygdell, JoakimAdriasola, MathildaEzcurra, InesTuominen, Hannele
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