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Efficient DNA-assisted synthesis of trans-membrane gold nanowires
KTH, Skolan för elektroteknik och datavetenskap (EECS), Mikro- och nanosystemteknik.ORCID-id: 0000-0003-4364-794X
Stockholm Univ, Dept Biochem & Biophys, Sci Life Lab, Tomtebodavagen 23 A, SE-17165 Solna, Sweden.
Stockholm Univ, Dept Biochem & Biophys, Sci Life Lab, Tomtebodavagen 23 A, SE-17165 Solna, Sweden.
Stockholm Univ, Dept Biochem & Biophys, Sci Life Lab, Tomtebodavagen 23 A, SE-17165 Solna, Sweden.
Vise andre og tillknytning
2018 (engelsk)Inngår i: Microsystems & Nanoengineering, ISSN 2055-7434, Vol. 4, s. 1-8, artikkel-id UNSP 17084Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Whereas electric circuits and surface-based (bio)chemical sensors are mostly constructed in-plane due to ease of manufacturing, 3D microscale and nanoscale structures allow denser integration of electronic components and improved mass transport of the analyte to (bio)chemical sensor surfaces. This work reports the first out-of-plane metallic nanowire formation based on stretching of DNA through a porous membrane. We use rolling circle amplification (RCA) to generate long single-stranded DNA concatemers with one end anchored to the surface. The DNA strands are stretched through the pores in the membrane during liquid removal by forced convection. Because the liquid–air interface movement across the membrane occurs in every pore, DNA stretching across the membrane is highly efficient. The stretched DNA molecules are transformed into trans-membrane gold nanowires through gold nanoparticle hybridization and gold enhancement chemistry. A 50 fM oligonucleotide concentration, a value two orders of magnitude lower than previously reported for flat surface-based nanowire formation, was sufficient for nanowire formation. We observed nanowires in up to 2.7% of the membrane pores, leading to an across-membrane electrical conductivity reduction from open circuit to o20 Ω. The simple electrical read-out offers a high signal-to-noise ratio and can also be extended for use as a biosensor due to the high specificity and scope for multiplexing offered by RCA.

sted, utgiver, år, opplag, sider
2018. Vol. 4, s. 1-8, artikkel-id UNSP 17084
HSV kategori
Identifikatorer
URN: urn:nbn:se:kth:diva-227196DOI: 10.1038/micronano.2017.84ISI: 000425451300001OAI: oai:DiVA.org:kth-227196DiVA, id: diva2:1203772
Forskningsfinansiär
Swedish Research Council, SBE13-0125Swedish Foundation for Strategic Research , SBE13-0125EU, Horizon 2020, 675412
Merknad

Correction in: Microsystems & Nanoengineering (2018) 4:9 DOI: 10.1038/s41378-018-0012-7, WOS: 000434457800001

QC 20180601

Tilgjengelig fra: 2018-05-04 Laget: 2018-05-04 Sist oppdatert: 2019-05-08bibliografisk kontrollert
Inngår i avhandling
1. Digital Electrical DNA Sensing
Åpne denne publikasjonen i ny fane eller vindu >>Digital Electrical DNA Sensing
2019 (engelsk)Doktoravhandling, med artikler (Annet vitenskapelig)
Abstract [en]

Molecule detection is a workhorse in life sciences and medicine, for example in cancer diagnosis and virus and bacterial detection. DNA analysis can provide vital information about the state of a host organism and its medical and health condition. A central challenge in DNA sensing lays in obtaining the following key detection characteristics in a single device: low limit of detection, small sample volume, high specificity, quantification, rapid time-to-result at a low cost.

Here we investigate whether direct electrical DNA sensing in a miniaturized detector can enable such performance. The detector consists of a gold-coated thin porous membrane, functionalized with oligonucleotides receptors, that is sandwiched between two off-stoichiometric thiol-ene-epoxy layers. The device works as follows. First, target DNA in the sample is specifically recognized by padlock probe hybridization and ligation. Second, the target-receptor circular molecules are amplified by rolling circle amplification (RCA), generating long ssDNA concatemers (RCP). Third, the RCPs are stretched through the membrane pores. Fourth, DNA metallization was used to form the gold nanowires bridging both sides of membrane pores after gold enhancement, which results in a conductive path that is measured with a simple resistance measurement. The thesis describes the engineering technology that enables low LoD detection of ssDNA using a digital measurement and details the development and optimization of the detector fabrication and operation, including structural design, materials, and microfluidic operation. We demonstrated a detector with sub-aM LoD, high specificity and simple operation in a miniaturized and uncomplicated format.

 Furthermore, the thesis studies the long-term liquid storage in nL scale well arrays fabricated in off-stoichiometric thiol-ene (OSTE). We demonstrated liquid storage with < 10 % loss of stored PBS buffer for 33 days and the on-demand electrically controlled liquid release.

The thesis presents the potential of a combination DNA detector with the method of liquid storage. Combining the on-chip liquid storage and DNA detection methods could provide a powerful alternative to conventional bio-detectors used in molecular diagnostics, and improved performance in multiplexed point-of-care sensing of (ultra-low abundant) biomolecules.

sted, utgiver, år, opplag, sider
Stockholm: KTH Royal Institute of Technology, 2019. s. 81
Serie
TRITA-EECS-AVL ; 2019:38
Emneord
DNA detection, thiol-ene-epoxy, rolling circle amplification, digital measurement, DNA stretching, microwell-array, gold nanowires, electrical measurement, specificity, limit of detection, liquid storage, disease diagnosis, lab-on-a-chip, point-of-care.
HSV kategori
Identifikatorer
urn:nbn:se:kth:diva-250783 (URN)978-91-7873-162-6 (ISBN)
Disputas
2019-06-11, Q2, Malvinas väg 10, Stockholm, 10:00 (engelsk)
Opponent
Veileder
Merknad

QC 20190508

Tilgjengelig fra: 2019-05-08 Laget: 2019-05-06 Sist oppdatert: 2019-05-14bibliografisk kontrollert

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