Ändra sökning
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Nanoscale localization of proteins within focal adhesions indicates discrete functional assemblies with selective force-dependence
KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Experimentell biomolekylär fysik.
Karolinska Inst, Dept Microbiol Tumor & Cell Biol, Stockholm, Sweden..
KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Experimentell biomolekylär fysik.
KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Kvant- och biofotonik.ORCID-id: 0000-0003-3200-0374
Visa övriga samt affilieringar
2018 (Engelska)Ingår i: The FEBS Journal, ISSN 1742-464X, E-ISSN 1742-4658, Vol. 285, nr 9, s. 1635-1652Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Focal adhesions (FAs) are subcellular regions at the micrometer scale that link the cell to the surrounding microenvironment and control vital cell functions. However, the spatial architecture of FAs remains unclear at the nanometer scale. We used two-color and three-color super-resolution stimulated emission depletion microscopy to determine the spatial distributions and co-localization of endogenous FA components in fibroblasts. Our data indicate that adhesion proteins inside, but not outside, FAs are organized into nanometer size units of multi-protein assemblies. The loss of contractile force reduced the nanoscale co-localization between different types of proteins, while it increased this co-localization between markers of the same type. This suggests that actomyosin-dependent force exerts a nonrandom, specific, control of the localization of adhesion proteins within cell-matrix adhesions. These observations are consistent with the possibility that proteins in cell-matrix adhesions are assembled in nanoscale particles, and that force regulates the localization of the proteins therein in a protein-specific manner. This detailed knowledge of how the organization of FA components at the nanometer scale is linked to the capacity of the cells to generate contractile forces expands our understanding of cell adhesion in health and disease.

Ort, förlag, år, upplaga, sidor
WILEY , 2018. Vol. 285, nr 9, s. 1635-1652
Nyckelord [en]
co-localization, focal adhesion-related particles, focal adhesions, quantitative imaging, stimulated emission depletion microscopy
Nationell ämneskategori
Cellbiologi
Identifikatorer
URN: urn:nbn:se:kth:diva-228269DOI: 10.1111/febs.14433ISI: 000431678400007PubMedID: 29542240Scopus ID: 2-s2.0-85044546393OAI: oai:DiVA.org:kth-228269DiVA, id: diva2:1209221
Anmärkning

QC 20180522

Tillgänglig från: 2018-05-22 Skapad: 2018-05-22 Senast uppdaterad: 2020-03-09Bibliografiskt granskad

Open Access i DiVA

Fulltext saknas i DiVA

Övriga länkar

Förlagets fulltextPubMedScopus

Personposter BETA

Rönnlund, DanielWidengren, Jerker

Sök vidare i DiVA

Av författaren/redaktören
Xu, LeiRönnlund, DanielWidengren, JerkerGad, Annica K. B.
Av organisationen
Experimentell biomolekylär fysikKvant- och biofotonik
I samma tidskrift
The FEBS Journal
Cellbiologi

Sök vidare utanför DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetricpoäng

doi
pubmed
urn-nbn
Totalt: 118 träffar
RefereraExporteraLänk till posten
Permanent länk

Direktlänk
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annat format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annat språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf