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Enzymatic Hydrolysis in the Green Production of Bacterial Cellulose Nanocrystals
Univ Milan, Dept Food Environm & Nutr Sci, DeFENS, Via Celoria 2, I-20133 Milan, Italy..
Univ Milan, Dept Food Environm & Nutr Sci, DeFENS, Via Celoria 2, I-20133 Milan, Italy..
Univ Milan, Dept Biosci, Via Celoria 26, I-20133 Milan, Italy..
Univ Milano Bicocca, Dept Mat Sci, Via R Cozzi 55, I-20125 Milan, Italy..
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2018 (English)In: ACS SUSTAINABLE CHEMISTRY & ENGINEERING, ISSN 2168-0485, Vol. 6, no 6, p. 7725-7734Article in journal (Refereed) Published
Abstract [en]

In this study, we extensively describe experimental models, with correlating experimental conditions, which were used to investigate the enzymatic hydrolysis of bacterial cellulose (BC) to obtain nanocrystals. Cellulase from Trichoderma reesei was used in five enzyme/BC ratios over a period of 74 h. The turbidity data was modeled using both logistic regression and empirical regression to determine the fractal kinetics, resulting in unique kinetic patterns for the mixtures that were richest in BC and in enzymes. The evolution of the yield was inversely related to the turbidity, as confirmed through a semiempirical approach that was adopted to model the experimental data. The yield values after 74 h of hydrolysis were higher for the substrate-rich mixtures (similar to 20%) than for the enzyme rich mixtures (similar to 5%), as corroborated by cellobiose and glucose quantification. Transmission electron microscopy and atomic force microscopy analyses revealed a shift from a fibril network to a needle-like morphology (i.e., aggregated nanocrystals or individual nanocrystals similar to 6 nm width and 200-800 nm in length) as the enzyme/BC ratios went from lower to higher. These results were explained in terms of the heterogeneous substrate model and the erosion model. This work initiated a promising, environmentally friendly method that could serve as an alternative to the commonly used chemical hydrolysis routes.

Place, publisher, year, edition, pages
AMER CHEMICAL SOC , 2018. Vol. 6, no 6, p. 7725-7734
Keywords [en]
atomic force microscopy, modeling, morphology, kinetic, transmission electron microscopy, turbidity, yield
National Category
Other Chemical Engineering
Identifiers
URN: urn:nbn:se:kth:diva-231195DOI: 10.1021/acssuschemeng.8b00600ISI: 000434491600059Scopus ID: 2-s2.0-85046765425OAI: oai:DiVA.org:kth-231195DiVA, id: diva2:1234010
Note

QC 20180720

Available from: 2018-07-20 Created: 2018-07-20 Last updated: 2018-07-20Bibliographically approved

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Olsson, Richard

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