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Calcium-dependent cyto- and genotoxicity of nickel metal and nickel oxide nanoparticles in human lung cells
Karolinska Inst, Inst Environm Med, Stockholm, Sweden..
Karolinska Inst, Inst Environm Med, Stockholm, Sweden..
Karolinska Inst, Inst Environm Med, Stockholm, Sweden..
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Chemistry, Surface and Corrosion Science.
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2018 (English)In: Particle and Fibre Toxicology, ISSN 1743-8977, E-ISSN 1743-8977, Vol. 15, article id 32Article in journal (Refereed) Published
Abstract [en]

Background: Genotoxicity is an important toxicological endpoint due to the link to diseases such as cancer. Therefore, an increased understanding regarding genotoxicity and underlying mechanisms is needed for assessing the risk with exposure to nanoparticles (NPs). The aim of this study was to perform an in-depth investigation regarding the genotoxicity of well-characterized Ni and NiO NPs in human bronchial epithelial BEAS-2B cells and to discern possible mechanisms. Comparisons were made with NiCl2 in order to elucidate effects of ionic Ni. Methods: BEAS-2B cells were exposed to Ni and NiO NPs, as well as NiCl2, and uptake and cellular dose were investigated by transmission electron microscopy (TEM) and inductively coupled plasma mass spectrometry (ICP-MS). The NPs were characterized in terms of surface composition (X-ray photoelectron spectroscopy), agglomeration (photon cross correlation spectroscopy) and nickel release in cell medium (ICP-MS). Cell death (necrosis/apoptosis) was investigated by Annexin VFITC/PI staining and genotoxicity by cytokinesis-block micronucleus (cytome) assay (OECD 487), chromosomal aberration (OECD 473) and comet assay. The involvement of intracellular reactive oxygen species (ROS) and calcium was explored using the fluorescent probes, DCFH-DA and Fluo-4. Results: NPs were efficiently taken up by the BEAS-2B cells. In contrast, no or minor uptake was observed for ionic Ni from NiCl2. Despite differences in uptake, all exposures (NiO, Ni NPs and NiCl2) caused chromosomal damage. Furthermore, NiO NPs were most potent in causing DNA strand breaks and generating intracellular ROS. An increase in intracellular calcium was observed and modulation of intracellular calcium by using inhibitors and chelators clearly prevented the chromosomal damage. Chelation of iron also protected against induced damage, particularly for NiO and NiCl2. Conclusions: This study has revealed chromosomal damage by Ni and NiO NPs as well as Ni ionic species and provides novel evidence for a calcium-dependent mechanism of cyto- and genotoxicity.

Place, publisher, year, edition, pages
BMC , 2018. Vol. 15, article id 32
Keywords [en]
Nickel/nickel oxide nanoparticles, Chromosomal aberrations, Endoreduplication, Calcium homeostasis, Carcinogenic potential
National Category
Pharmacology and Toxicology
Identifiers
URN: urn:nbn:se:kth:diva-232767DOI: 10.1186/s12989-018-0268-yISI: 000439340800001PubMedID: 30016969Scopus ID: 2-s2.0-85050147056OAI: oai:DiVA.org:kth-232767DiVA, id: diva2:1236599
Funder
Forte, Swedish Research Council for Health, Working Life and Welfare, 2011-0832Swedish Research Council, 2014-4598 2017-03931
Note

QC 20180803

Available from: 2018-08-03 Created: 2018-08-03 Last updated: 2019-08-20Bibliographically approved

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Skoglund, SaraOdnevall Wallinder, Inger

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