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BARM and BalticMicrobeDB, a reference metagenome and interface to meta-omic data for the Baltic Sea
KTH, Skolan för kemi, bioteknologi och hälsa (CBH), Genteknologi. KTH, Centra, Science for Life Laboratory, SciLifeLab.
Stockholm Univ, Sci Life Lab, Dept Biochem & Biophys, S-17165 Solna, Sweden..
Leibniz Inst Balt Sea Res Warnemunde, D-18119 Rostock, Germany..
Leibniz Inst Balt Sea Res Warnemunde, D-18119 Rostock, Germany..
Vise andre og tillknytning
2018 (engelsk)Inngår i: Scientific Data, E-ISSN 2052-4463, Vol. 5, artikkel-id 180146Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

The Baltic Sea is one of the world's largest brackish water bodies and is characterised by pronounced physicochemical gradients where microbes are the main biogeochemical catalysts. Meta-omic methods provide rich information on the composition of, and activities within, microbial ecosystems, but are computationally heavy to perform. We here present the Baltic Sea Reference Metagenome (BARM), complete with annotated genes to facilitate further studies with much less computational effort. The assembly is constructed using 2.6 billion metagenomic reads from 81 water samples, spanning both spatial and temporal dimensions, and contains 6.8 million genes that have been annotated for function and taxonomy. The assembly is useful as a reference, facilitating taxonomic and functional annotation of additional samples by simply mapping their reads against the assembly. This capability is demonstrated by the successful mapping and annotation of 24 external samples. In addition, we present a public web interface, BalticMicrobeDB, for interactive exploratory analysis of the dataset.

sted, utgiver, år, opplag, sider
Nature Publishing Group , 2018. Vol. 5, artikkel-id 180146
HSV kategori
Identifikatorer
URN: urn:nbn:se:kth:diva-233290DOI: 10.1038/sdata.2018.146ISI: 000440291200001PubMedID: 30063227Scopus ID: 2-s2.0-85051806706OAI: oai:DiVA.org:kth-233290DiVA, id: diva2:1239392
Forskningsfinansiär
Swedish Research Council, 2011-5689Science for Life Laboratory - a national resource center for high-throughput molecular bioscience
Merknad

QC 20180816

Tilgjengelig fra: 2018-08-16 Laget: 2018-08-16 Sist oppdatert: 2019-10-09bibliografisk kontrollert

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Alneberg, JohannesAndersson, Anders F.

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