Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Acoustic formation of multicellular tumor spheroids enabling on-chip functional and structural imaging
KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Biomedicinsk fysik och röntgenfysik.
Karolinska Inst, Dept Microbiol Tumor & Cell Biol, Stockholm, Sweden..
KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Biomedicinsk fysik och röntgenfysik.ORCID-id: 0000-0002-7023-4772
KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Biomedicinsk fysik och röntgenfysik.
Vise andre og tillknytning
2018 (engelsk)Inngår i: Lab on a Chip, ISSN 1473-0197, E-ISSN 1473-0189, Vol. 18, nr 16, s. 2466-2476Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Understanding the complex 3D tumor microenvironment is important in cancer research. This microenvironment can be modelled in vitro by culturing multicellular tumor spheroids (MCTS). Key challenges when using MCTS in applications such as high-throughput drug screening are overcoming imaging and analytical issues encountered during functional and structural investigations. To address these challenges, we use an ultrasonic standing wave (USW) based MCTS culture platform for parallel formation, staining and imaging of 100 whole MCTS. A protein repellent amphiphilic polymer coating enables flexible production of high quality and unanchored MCTS. This enables high-content multimode analysis based on flow cytometry and in situ optical microscopy. We use HepG2 hepatocellular carcinoma, A498 and ACHN renal carcinoma, and LUTC-2 thyroid carcinoma cell lines to demonstrate (i) the importance of the ultrasound-coating combination, (ii) bright field image based automatic characterization of MTCS, (iii) detailed deep tissue confocal imaging of whole MCTS mounted in a refractive index matching solution, and (iv) single cell functional analysis through flow cytometry of single cell suspensions of disintegrated MTCS. The USW MCTS culture platform is customizable and holds great potential for detailed multimode MCTS analysis in a high-content manner.

sted, utgiver, år, opplag, sider
Royal Society of Chemistry, 2018. Vol. 18, nr 16, s. 2466-2476
HSV kategori
Identifikatorer
URN: urn:nbn:se:kth:diva-234606DOI: 10.1039/c8lc00537kISI: 000442265100012PubMedID: 30033460Scopus ID: 2-s2.0-85051357097OAI: oai:DiVA.org:kth-234606DiVA, id: diva2:1248230
Forskningsfinansiär
Stockholm County CouncilSwedish Cancer SocietySwedish Childhood Cancer FoundationSwedish Research CouncilSwedish Foundation for Strategic Research
Merknad

QC 20180914

Tilgjengelig fra: 2018-09-14 Laget: 2018-09-14 Sist oppdatert: 2018-09-14bibliografisk kontrollert

Open Access i DiVA

Fulltekst mangler i DiVA

Andre lenker

Forlagets fulltekstPubMedScopus

Personposter BETA

Olofsson, KarlOhlin, MathiasFrisk, ThomasÖnfelt, BjörnWiklund, Martin

Søk i DiVA

Av forfatter/redaktør
Olofsson, KarlOhlin, MathiasFrisk, ThomasÖnfelt, BjörnWiklund, Martin
Av organisasjonen
I samme tidsskrift
Lab on a Chip

Søk utenfor DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric

doi
pubmed
urn-nbn
Totalt: 43 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf