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Acoustic formation of multicellular tumor spheroids enabling on-chip functional and structural imaging
KTH, School of Engineering Sciences (SCI), Applied Physics, Biomedical and X-ray Physics.
Karolinska Inst, Dept Microbiol Tumor & Cell Biol, Stockholm, Sweden..
KTH, School of Engineering Sciences (SCI), Applied Physics, Biomedical and X-ray Physics.ORCID iD: 0000-0002-7023-4772
KTH, School of Engineering Sciences (SCI), Applied Physics, Biomedical and X-ray Physics.
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2018 (English)In: Lab on a Chip, ISSN 1473-0197, E-ISSN 1473-0189, Vol. 18, no 16, p. 2466-2476Article in journal (Refereed) Published
Abstract [en]

Understanding the complex 3D tumor microenvironment is important in cancer research. This microenvironment can be modelled in vitro by culturing multicellular tumor spheroids (MCTS). Key challenges when using MCTS in applications such as high-throughput drug screening are overcoming imaging and analytical issues encountered during functional and structural investigations. To address these challenges, we use an ultrasonic standing wave (USW) based MCTS culture platform for parallel formation, staining and imaging of 100 whole MCTS. A protein repellent amphiphilic polymer coating enables flexible production of high quality and unanchored MCTS. This enables high-content multimode analysis based on flow cytometry and in situ optical microscopy. We use HepG2 hepatocellular carcinoma, A498 and ACHN renal carcinoma, and LUTC-2 thyroid carcinoma cell lines to demonstrate (i) the importance of the ultrasound-coating combination, (ii) bright field image based automatic characterization of MTCS, (iii) detailed deep tissue confocal imaging of whole MCTS mounted in a refractive index matching solution, and (iv) single cell functional analysis through flow cytometry of single cell suspensions of disintegrated MTCS. The USW MCTS culture platform is customizable and holds great potential for detailed multimode MCTS analysis in a high-content manner.

Place, publisher, year, edition, pages
Royal Society of Chemistry, 2018. Vol. 18, no 16, p. 2466-2476
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
URN: urn:nbn:se:kth:diva-234606DOI: 10.1039/c8lc00537kISI: 000442265100012PubMedID: 30033460Scopus ID: 2-s2.0-85051357097OAI: oai:DiVA.org:kth-234606DiVA, id: diva2:1248230
Funder
Stockholm County CouncilSwedish Cancer SocietySwedish Childhood Cancer FoundationSwedish Research CouncilSwedish Foundation for Strategic Research
Note

QC 20180914

Available from: 2018-09-14 Created: 2018-09-14 Last updated: 2018-09-14Bibliographically approved

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Olofsson, KarlOhlin, MathiasFrisk, ThomasÖnfelt, BjörnWiklund, Martin

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Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

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