Tailormade Polysaccharides with Defined Branching Patterns: Enzymatic Polymerization of Arabinoxylan OligosaccharidesShow others and affiliations
2018 (English)In: Angewandte Chemie International Edition, ISSN 1433-7851, E-ISSN 1521-3773, Vol. 57, no 37, p. 11987-11992Article in journal (Refereed) Published
Abstract [en]
The heterogeneous nature of non-cellulosic polysaccharides, such as arabinoxylan, makes it difficult to correlate molecular structure with macroscopic properties. To study the impact of specific structural features of the polysaccharides on crystallinity or affinity to other cell wall components, collections of polysaccharides with defined repeating units are required. Herein, a chemoenzymatic approach to artificial arabinoxylan polysaccharides with systematically altered branching patterns is described. The polysaccharides were obtained by glycosynthase-catalyzed polymerization of glycosyl fluorides derived from arabinoxylan oligosaccharides. X-ray diffraction and adsorption experiments on cellulosic surfaces revealed that the physicochemical properties of the synthetic polysaccharides strongly depend on the specific nature of their substitution patterns. The artificial polysaccharides allow structure-property relationship studies that are not accessible by other means.
Place, publisher, year, edition, pages
Wiley-VCH Verlagsgesellschaft, 2018. Vol. 57, no 37, p. 11987-11992
Keywords [en]
carbohydrates, enzymes, glycosynthases, structure elucidation, synthetic methods
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:kth:diva-235104DOI: 10.1002/anie.201806871ISI: 000443675700024PubMedID: 30044516Scopus ID: 2-s2.0-85052657815OAI: oai:DiVA.org:kth-235104DiVA, id: diva2:1248757
Funder
Knut and Alice Wallenberg Foundation
Note
QC 20180917
2018-09-172018-09-172024-03-18Bibliographically approved
In thesis