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Chemical characterization and wound healing property of a beta-D-glucan from edible mushroom Piptoporus betulinus
Univ Fed Parana, Dept Biochem & Mol Biol, CP 19046, Curitiba, PR, Brazil..
Univ Fed Parana, Dept Biochem & Mol Biol, CP 19046, Curitiba, PR, Brazil..
KTH, Skolan för kemi, bioteknologi och hälsa (CBH), Kemi, Glykovetenskap.ORCID-id: 0000-0002-8533-1022
KTH, Skolan för kemi, bioteknologi och hälsa (CBH), Fiber- och polymerteknologi.ORCID-id: 0000-0003-3572-7798
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2018 (Engelska)Ingår i: International Journal of Biological Macromolecules, ISSN 0141-8130, E-ISSN 1879-0003, Vol. 117, s. 1361-1366Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

A water-soluble beta-D-glucan was obtained from fruiting bodies of Piptoporus betulinus, by hot aqueous extraction followed by freeze-thawing procedure and dialysis. Its molar mass distribution and conformational behavior in solution was assessed by size-exclusion chromatography coupled with multiangle laser light scattering, showing a polysaccharide with an average molecular weight of 2.5 x 10(5) Da with a random coil conformation for molecular weights below 1 x 10(6) Da. Typical signals of beta-(1 -> 3)-linkages were observed in NMR spectrum (delta 102.7/4.76; 102.8/4.74; 102.9/4.52; and delta 85.1/3.78; 85.0/3.77) and also signals of O-6 substitution at delta 69.2/4.22 and 69.2/3.87. The analysis of partially O-methylated alditol acetates corroborates the NMR results, indicating the presence of a beta-D-glucan with a main chain (1 -> 3)-linked, substituted at O-6 by single-units of glucose. The beta-D-glucan showed no toxicity on human colon carcinoma cell line (Caco-2) up to 1000 mu g mL(-1) and promoted cell migration on in vitro scratch assay, demonstrating a potential wound healing capacity.

Ort, förlag, år, upplaga, sidor
Elsevier, 2018. Vol. 117, s. 1361-1366
Nyckelord [en]
beta-D-glucan, Random coil, Wound healing
Nationell ämneskategori
Cell- och molekylärbiologi
Identifikatorer
URN: urn:nbn:se:kth:diva-234561DOI: 10.1016/j.ijbiomac.2017.12.107ISI: 000442057700154PubMedID: 29274425Scopus ID: 2-s2.0-85042161770OAI: oai:DiVA.org:kth-234561DiVA, id: diva2:1249465
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QC 20180919

Tillgänglig från: 2018-09-19 Skapad: 2018-09-19 Senast uppdaterad: 2018-11-13Bibliografiskt granskad

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Ruthes, Andrea C.Vilaplana, Francisco

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Ruthes, Andrea C.Vilaplana, Francisco
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GlykovetenskapFiber- och polymerteknologi
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International Journal of Biological Macromolecules
Cell- och molekylärbiologi

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