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Autoantibody profiling reveals four protein candidate autoantigens associated with systemic lupus erythematosus
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Protein Science.ORCID iD: 0000-0003-0880-5375
KTH, Centres, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0002-4657-8532
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2018 (English)In: Lupus, ISSN 0961-2033, E-ISSN 1477-0962, Vol. 27, no 10, p. 1670-1678Article in journal (Refereed) Published
Abstract [en]

Objectives In systemic lupus erythematosus (SLE) there are typically many autoantibodies. The disease heterogeneity could be better understood with discovery of phenotype-specific antigens targeted by autoantibodies. We here aimed to identify novel autoantigens potentially related to SLE disease and a major complication, atherosclerosis. Methods Antigen microarrays were used to profile IgG autoantibody reactivity against 77 protein fragments (20-140 amino acids (aa) long, median 89 aa) produced within the Human Protein Atlas project, in serum samples from SLE patients (n=107) and age- and sex-matched population-based controls (n=107). Common carotid intima-media thickness, plaque occurrence and echogenicity were determined by B-mode ultrasound. Results We determined significant differences between patients and controls in IgG reactivity against four proteins. In patients compared to controls, there was an increase of IgG reactivity against zinc finger protein 688 (ZNF688), early B cell factor 2 (EBF2), crystallin, alpha B (CRYAB) and tumor necrosis factor receptor superfamily member 13C (TNFRSF13C). Of these four antigens, only anti-ZNF688 was associated with carotid atherosclerosis (plaque occurrence) and vulnerable plaques in SLE. There was a weak association between anti-EBF2 and SLE disease activity but no significant associations were determined for other measured IgG reactivity. Conclusions In this discovery screening we here demonstrate new candidate autoantigens with differential reactivity (reflecting autoantibody levels) in SLE patients and in controls and in relation to atherosclerosis in SLE.

Place, publisher, year, edition, pages
SAGE PUBLICATIONS LTD , 2018. Vol. 27, no 10, p. 1670-1678
Keywords [en]
Systemic lupus erythematosus, autoantigen, protein array, atherosclerosis, antibodies
National Category
Rheumatology and Autoimmunity
Identifiers
URN: urn:nbn:se:kth:diva-234163DOI: 10.1177/0961203318788153ISI: 000441284400011PubMedID: 30041579Scopus ID: 2-s2.0-85051491643OAI: oai:DiVA.org:kth-234163DiVA, id: diva2:1257271
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QC 20181019

Available from: 2018-10-19 Created: 2018-10-19 Last updated: 2018-11-12Bibliographically approved

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Hellström, CeciliaNilsson, Peter

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