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High-density antigen microarrays for the assessment of antibody selectivity and off-target binding
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Protein Science, Affinity Proteomics. KTH, Centres, Science for Life Laboratory, SciLifeLab.
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Protein Science, Affinity Proteomics. KTH, Centres, Science for Life Laboratory, SciLifeLab.
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Protein Science, Affinity Proteomics. KTH, Centres, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0003-0880-5375
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Protein Science, Affinity Proteomics. KTH, Centres, Science for Life Laboratory, SciLifeLab.
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2018 (English)In: Epitope Mapping Protocols, Humana Press Inc. , 2018, p. 231-238Chapter in book (Refereed)
Abstract [en]

With the increasing availability of collections of antibodies, their evaluation in terms of binding selectivity becomes an important but challenging task. Planar antigen microarrays are very suitable tools to address this task and provide a powerful proteomics platform for the characterization of the binding selectivity of antibodies toward thousands of antigens in parallel. In this chapter, we describe our in-house developed procedures for the generation of high-density planar antigen microarrays with over 21,000 features. We also provide the details of the assay protocol, which we routinely use for the assessment of binding selectivity of the polyclonal antibodies generated within the Human Protein Atlas. © Springer Science+Business Media, LLC, part of Springer Nature 2018.

Place, publisher, year, edition, pages
Humana Press Inc. , 2018. p. 231-238
Series
Methods in Molecular Biology, ISSN 1064-3745 ; 1785
Keywords [en]
Affinity proteomics, Antibody selectivity, Antigen microarrays, Protein microarrays
National Category
Other Industrial Biotechnology
Identifiers
URN: urn:nbn:se:kth:diva-238338DOI: 10.1007/978-1-4939-7841-0_15Scopus ID: 2-s2.0-85046366884ISBN: 978-1-4939-7839-7 (print)OAI: oai:DiVA.org:kth-238338DiVA, id: diva2:1263340
Funder
Science for Life Laboratory - a national resource center for high-throughput molecular bioscience
Note

QC 20181115

Available from: 2018-11-15 Created: 2018-11-15 Last updated: 2019-10-08Bibliographically approved

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Hellström, CeciliaSchwenk, Jochen M.Nilsson, PeterAyoglu, Burcu

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Sjöberg, RonaldAndersson, EniHellström, CeciliaMattsson, CeciliaSchwenk, Jochen M.Nilsson, PeterAyoglu, Burcu
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