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Multiplexed antigen bead arrays for the assessment of antibody selectivity and epitope mapping
KTH, Skolan för kemi, bioteknologi och hälsa (CBH), Proteinvetenskap, Affinity Proteomics. KTH, Centra, Science for Life Laboratory, SciLifeLab.ORCID-id: 0000-0001-7843-2960
KTH, Skolan för kemi, bioteknologi och hälsa (CBH), Proteinvetenskap, Affinity Proteomics. KTH, Centra, Science for Life Laboratory, SciLifeLab.ORCID-id: 0000-0002-4657-8532
KTH, Skolan för kemi, bioteknologi och hälsa (CBH), Proteinvetenskap, Affinity Proteomics. KTH, Centra, Science for Life Laboratory, SciLifeLab.ORCID-id: 0000-0001-8141-8449
2018 (engelsk)Inngår i: Epitope Mapping Protocols, Humana Press Inc. , 2018, s. 239-248Kapittel i bok, del av antologi (Fagfellevurdert)
Abstract [en]

With the increasing number of binding reagents for affinity-based investigations of the human proteome, high-throughput tools for the characterization of the used reagents become essential. For the analysis of binding selectivity, bead-based antigen arrays offer a miniaturized and parallelized assay platform to meet such needs, as they enable two-dimensional multiplexing to analyze up to 384 samples against up to 500 analytes in a single round of analysis. In this chapter, we describe our protocols for the generation of multiplex bead arrays built on immobilized protein fragments, as well as biotinylated peptides. Combined together, these two versions of antigen arrays offer a versatile approach for multiplexed characterization of antibody binding selectivity, off-target interactions, as well as mapping for the amino acids of epitopes involved in antibody binding.

sted, utgiver, år, opplag, sider
Humana Press Inc. , 2018. s. 239-248
Serie
Methods in Molecular Biology, ISSN 1064-3745 ; 1785
Emneord [en]
Affinity proteomics, Antibody selectivity, Antigen arrays, Epitope mapping, Peptide arrays, Protein arrays, Suspension bead arrays
HSV kategori
Identifikatorer
URN: urn:nbn:se:kth:diva-238377DOI: 10.1007/978-1-4939-7841-0_16Scopus ID: 2-s2.0-85046341154OAI: oai:DiVA.org:kth-238377DiVA, id: diva2:1264882
Forskningsfinansiär
Science for Life Laboratory - a national resource center for high-throughput molecular bioscience
Merknad

QC 20181121

Tilgjengelig fra: 2018-11-21 Laget: 2018-11-21 Sist oppdatert: 2018-11-21bibliografisk kontrollert

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Ayoglu, BurcuNilsson, PeterSchwenk, Jochen M.

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