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Stronger cellulose microfibrils network structure through the expression of cellulose-binding modules in plant primary cell walls
KTH, Skolan för kemi, bioteknologi och hälsa (CBH), Kemi, Glykovetenskap.ORCID-id: 0000-0002-5541-7853
KTH, Skolan för kemi, bioteknologi och hälsa (CBH), Kemi, Glykovetenskap.
KTH, Skolan för kemi, bioteknologi och hälsa (CBH), Kemi, Glykovetenskap.ORCID-id: 0000-0001-9832-027X
(engelsk)Manuskript (preprint) (Annet vitenskapelig)
HSV kategori
Identifikatorer
URN: urn:nbn:se:kth:diva-240966OAI: oai:DiVA.org:kth-240966DiVA, id: diva2:1275526
Merknad

QC 20190107

Tilgjengelig fra: 2019-01-07 Laget: 2019-01-07 Sist oppdatert: 2019-05-08bibliografisk kontrollert
Inngår i avhandling
1.
Posten ble ikke funnet. Det kan skyldes at posten ikke lenger er tilgjengelig eller det er feil id i adressefeltet.
2. Understanding and manipulating primary cell walls in plant cell suspension cultures
Åpne denne publikasjonen i ny fane eller vindu >>Understanding and manipulating primary cell walls in plant cell suspension cultures
2019 (engelsk)Doktoravhandling, med artikler (Annet vitenskapelig)
Abstract [en]

The cell wall is required for many aspects of plant function and development. It is also an accessible and renewable resource utilized both in unrefined forms and as raw material for further development. Increased knowledge regarding cell wall structure and components will contribute to better utilization of plants and the resources they provide. In this thesis aspects of the primary cell wall of Populus trichocarpa and Nicotiana tabacum are explored.

In Publication I a method for isolation and biochemical characterization of plant glycosyltransferases using a spectrophotometric or a radiometric assay was optimized. The radiometric assay was applied in Publication II where the proteome of the plasmodesmata isolated from P. trichocarpa was analyzed. Proteins identified belonged to functional classes such as “transport”, “signalling” and “stress responses”. Plasmodesmata-enriched fractions had high levels of callose synthase activity under ion depleted conditions as well as with calcium present.

The second part of the thesis comprises the alteration of the cell wall of N. tabacum cells and A. thaliana plants through in vivo expression of a carbohydrate binding module (CBM) (Publication III). In tobacco this resulted in cell walls with loose ultrastructure containing an increased proportion of 1,4-β-glucans. The cell walls were more susceptible to saccharification, possibly due to changes in the structure of cellulose or xyloglucan. Arabidopsis plants showed increased saccharification after mild pretreatment, suggesting that heterologous expression of CBMs is a promising method for cell wall engineering. In Publication IV cellulose microfibrils (CMFs) and nanocrystals (CNCs) were extracted from the transgenic cells. CNC preparation resulted in higher yields and longer CNCs. Nanopapers prepared from the CMFs of the CBM line demonstrated enhanced strength and toughness. Thus, changes to the ordered regions of cellulose were suggested to take place due to CBM expression.

sted, utgiver, år, opplag, sider
Stockholm: KTH Royal Institute of Technology, 2019. s. 82
Serie
TRITA-CBH-FOU ; 2
Emneord
Callose synthase, carbohydrate-binding module, cell wall engineering, cellulose microfibril, cellulose nanocrystal, glycosyltransferase, mass spectrometry, plasmodesmata, Populus, primary cell wall, radiometric assay, spectrophotometric assay
HSV kategori
Forskningsprogram
Bioteknologi
Identifikatorer
urn:nbn:se:kth:diva-251039 (URN)978-91-7873-074-2 (ISBN)
Disputas
2019-06-10, FA32, Roslagstullsbacken 21, AlbaNova, Stockholm, 10:00 (engelsk)
Opponent
Veileder
Merknad

QC 2019-05-08

Tilgjengelig fra: 2019-05-08 Laget: 2019-05-08 Sist oppdatert: 2019-05-08bibliografisk kontrollert

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