Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Graphene Oxide Tablets for Sample Preparation of Drugs in Biological Fluids: Determination of Omeprazole in Human Saliva for Liquid Chromatography Tandem Mass Spectrometry
KTH, School of Engineering Sciences (SCI), Applied Physics. Karolinska Inst, Dept Clin Neurosci, Ctr Psychiat Res, SE-17176 Solna, Sweden ; Univ Bu Ali Sina, Dept Chem, Hamadan 65174, Iran.
Univ Bu Ali Sina, Dept Chem, Hamadan 65174, Iran..
KTH, School of Engineering Sciences (SCI), Applied Physics.
Karolinska Inst, Dept Clin Neurosci, Ctr Psychiat Res, SE-17176 Solna, Sweden..
Show others and affiliations
2019 (English)In: Molecules, ISSN 1420-3049, E-ISSN 1420-3049, Vol. 24, no 7, article id 1191Article in journal (Refereed) Published
Abstract [en]

In this study, a novel sort of sample preparation sorbent was developed, by preparing thin layer graphene oxide tablets (GO-Tabs) utilizing a mixture of graphene oxide and polyethylene glycol on a polyethylene substrate. The GO-Tabs were used for extraction and concentration of omeprazole (OME) in human saliva samples. The determination of OME was carried out using liquid chromatography-tandem mass spectrometry (LC-MS/MS) under gradient LC conditions and in the positive ion mode (ESI+) with mass transitions of m/z 346.3 -> 198.0 for OME and m/z 369.98 -> 252.0 for the internal standard. Standard calibration for the saliva samples was in the range of 2.0-2000 nmol L-1. Limits of detection and quantification were 0.05 and 2.0 nmol L-1, respectively. Method validation showed good method accuracy and precision; the inter-day precision values ranged from 5.7 to 8.3 (%RSD), and the accuracy of determinations varied from -11.8% to 13.3% (% deviation from nominal values). The extraction recovery was 60%, and GO-Tabs could be re-used for more than ten extractions without deterioration in recovery. In this study, the determination of OME in real human saliva samples using GO-Tab extraction was validated.

Place, publisher, year, edition, pages
MDPI , 2019. Vol. 24, no 7, article id 1191
Keywords [en]
graphene oxide, omeprazole, liquid chromatography, tandem mass spectrometry, saliva, GO-Tabs
National Category
Biophysics
Identifiers
URN: urn:nbn:se:kth:diva-251343DOI: 10.3390/molecules24071191ISI: 000464946300002PubMedID: 30934659Scopus ID: 2-s2.0-85063759590OAI: oai:DiVA.org:kth-251343DiVA, id: diva2:1317081
Note

QC 20190521

Available from: 2019-05-21 Created: 2019-05-21 Last updated: 2019-05-21Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMedScopus

Authority records BETA

Zohdi, ZeynabUheida, AbdusalamAbdel-Rehim, Mohamed

Search in DiVA

By author/editor
Zohdi, ZeynabUheida, AbdusalamAbdel-Rehim, Mohamed
By organisation
Applied Physics
In the same journal
Molecules
Biophysics

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 26 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • harvard1
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf