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Electrospray sample injection for single-particle imaging with x-ray lasers
Uppsala Univ, Dept Cell & Mol Biol, Lab Mol Biophys, Husargatan 3,Box 596, SE-75124 Uppsala, Sweden.;European XFEL GmbH, Holzkoppel 4, D-22869 Schenefeld, Germany..
KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Biomedicinsk fysik och röntgenfysik. Uppsala Univ, Dept Cell & Mol Biol, Lab Mol Biophys, Husargatan 3,Box 596, SE-75124 Uppsala, Sweden.
KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Biomedicinsk fysik och röntgenfysik. Uppsala Univ, Dept Cell & Mol Biol, Lab Mol Biophys, Husargatan 3,Box 596, SE-75124 Uppsala, Sweden.ORCID-id: 0000-0003-2793-5052
Uppsala Univ, Dept Cell & Mol Biol, Lab Mol Biophys, Husargatan 3,Box 596, SE-75124 Uppsala, Sweden.;Lawrence Berkeley Natl Lab, NERSC, Berkeley, CA 94720 USA..
Rekke forfattare: 392019 (engelsk)Inngår i: Science Advances, E-ISSN 2375-2548, Vol. 5, nr 5, artikkel-id eaav8801Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

The possibility of imaging single proteins constitutes an exciting challenge for x-ray lasers. Despite encouraging results on large particles, imaging small particles has proven to be difficult for two reasons: not quite high enough pulse intensity from currently available x-ray lasers and, as we demonstrate here, contamination of the aerosolized molecules by nonvolatile contaminants in the solution. The amount of contamination on the sample depends on the initial droplet size during aerosolization. Here, we show that, with our electrospray injector, we can decrease the size of aerosol droplets and demonstrate virtually contaminant-free sample delivery of organelles, small virions, and proteins. The results presented here, together with the increased performance of next-generation x-ray lasers, constitute an important stepping stone toward the ultimate goal of protein structure determination from imaging at room temperature and high temporal resolution.

sted, utgiver, år, opplag, sider
American Association for the Advancement of Science , 2019. Vol. 5, nr 5, artikkel-id eaav8801
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URN: urn:nbn:se:kth:diva-254114DOI: 10.1126/sciadv.aav8801ISI: 000470125000080PubMedID: 31058226Scopus ID: 2-s2.0-85065606358OAI: oai:DiVA.org:kth-254114DiVA, id: diva2:1328982
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QC 20190624

Tilgjengelig fra: 2019-06-24 Laget: 2019-06-24 Sist oppdatert: 2019-06-24bibliografisk kontrollert

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