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An Autonomous Microfluidic Device for Generating Volume-Defined Dried Plasma Spots
KTH, School of Electrical Engineering and Computer Science (EECS), Micro and Nanosystems.ORCID iD: 0000-0003-3601-0342
KTH, School of Electrical Engineering and Computer Science (EECS), Micro and Nanosystems.
Karolinska Univ Hosp, Clin Pharmacol, S-11486 Stockholm, Sweden..
Karolinska Univ Hosp, Clin Pharmacol, S-11486 Stockholm, Sweden..
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2019 (English)In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 91, no 11, p. 7125-7130Article in journal (Refereed) Published
Abstract [en]

Obtaining plasma from a blood sample and preparing it for subsequent analysis is currently a laborious process involving experienced health-care professionals and centrifugation. We circumvent this by utilizing capillary forces and microfluidic engineering to develop an autonomous plasma sampling device that filters and stores an exact amount of plasma as a dried plasma spot (DPS) from a whole blood sample in less than 6 min. We tested 24 prototype devices with whole blood from 10 volunteers, various input volumes (40-80 mu L), and different hematocrit levels (39-45%). The resulting mean plasma volume, assessed gravimetrically, was 11.6 mu L with a relative standard deviation similar to manual pipetting (3.0% vs 1.4%). LC-MS/MS analysis of caffeine concentrations in the generated DPS (12 duplicates) showed a strong correlation (R-2 = 0.99) to, but no equivalence with, concentrations prepared from corresponding plasma obtained by centrifugation. The presented autonomous DPS device may enable patient-centric plasma sampling through minimally invasive finger-pricking and allow generatation of volume-defined DPS for quantitative blood analysis.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2019. Vol. 91, no 11, p. 7125-7130
National Category
Biomedical Laboratory Science/Technology
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URN: urn:nbn:se:kth:diva-254080DOI: 10.1021/acs.analchem.9b00204ISI: 000470793800027PubMedID: 31063366Scopus ID: 2-s2.0-85066116426OAI: oai:DiVA.org:kth-254080DiVA, id: diva2:1330278
Note

QC 20190625

Available from: 2019-06-25 Created: 2019-06-25 Last updated: 2019-06-25Bibliographically approved

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Hauser, JanoschLenk, GabrielStemme, GöranRoxhed, Niclas

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