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A collagen-based microwell migration assay to study NK-target cell interactions
KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Biofysik. KTH, Centra, Science for Life Laboratory, SciLifeLab.ORCID-id: 0000-0002-6019-8157
KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Biofysik. KTH, Centra, Science for Life Laboratory, SciLifeLab.ORCID-id: 0000-0003-2040-3176
KTH, Skolan för elektro- och systemteknik (EES), Signalbehandling. KTH, Skolan för elektroteknik och datavetenskap (EECS), Centra, ACCESS Linnaeus Centre.ORCID-id: 0000-0002-5329-575X
KTH, Skolan för teknikvetenskap (SCI), Tillämpad fysik, Biofysik. KTH, Centra, Science for Life Laboratory, SciLifeLab.
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2019 (engelsk)Inngår i: Scientific Reports, ISSN 2045-2322, E-ISSN 2045-2322, Vol. 9, artikkel-id 10672Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

Natural killer (NK) cell cytotoxicity in tissue is dependent on the ability of NK cells to migrate through the extracellular matrix (ECM) microenvironment. Traditional imaging studies of NK cell migration and cytotoxicity have utilized 2D surfaces, which do not properly reproduce the structural and mechanical cues that shape the migratory response of NK cells in vivo. Here, we have combined a microwell assay that allows long-term imaging and tracking of small, well-defined populations of NK cells with an interstitial ECM-like matrix. The assay allows for long-term imaging of NK-target cell interactions within a confined 3D volume. We found marked differences in motility between individual cells with a small fraction of the cells moving slowly and being confined to a small volume within the matrix, while other cells moved more freely. A majority of NK cells also exhibited transient variation in their motility, alternating between periods of migration arrest and movement. The assay could be used as a complement to in vivo imaging to study human NK cell heterogeneity in migration and cytotoxicity.

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Nature Publishing Group, 2019. Vol. 9, artikkel-id 10672
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URN: urn:nbn:se:kth:diva-255740DOI: 10.1038/s41598-019-46958-3ISI: 000476718900058PubMedID: 31337806Scopus ID: 2-s2.0-85069667997OAI: oai:DiVA.org:kth-255740DiVA, id: diva2:1341937
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QC 20190812

Tilgjengelig fra: 2019-08-12 Laget: 2019-08-12 Sist oppdatert: 2019-08-12bibliografisk kontrollert

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Olofsson, Per E.Brandt, LudwigMagnusson, Klas E. G.Frisk, ThomasJaldén, JoakimÖnfelt, Björn

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