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Nativity of lignin carbohydrate bonds substantiated by biomimetic synthesis
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Fibre- and Polymer Technology. KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Centres, Wallenberg Wood Science Center.ORCID iD: 0000-0003-4266-0720
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2019 (English)In: Journal of Experimental Botany, ISSN 0022-0957, E-ISSN 1460-2431Article in journal (Refereed) Published
Abstract [en]

The question of whether lignin is covalently linked to carbohydrates in native wood, forming what is referred to as lignin–carbohydrate complexes (LCCs), still lacks unequivocal proof. This is mainly due to the need to isolate lignin from woody materials prior to analysis, under conditions leading to partial chemical modification of the native wood polymers. Thus, the correlation between the structure of the isolated LCCs and LCCs in situ remains open. As a way to circumvent the problematic isolation, biomimicking lignin polymerization in vivo and in vitro is an interesting option. Herein, we report the detection of lignin–carbohydrate bonds in the extracellular lignin formed by tissue-cultured Norway spruce cells, and in modified biomimetic lignin synthesis (dehydrogenation polymers). Semi-quantitative 2D heteronuclear singular quantum coherence (HSQC)-, 31P -, and 13C-NMR spectroscopy were applied as analytical tools. Combining results from these systems, four types of lignin–carbohydrate bonds were detected; benzyl ether, benzyl ester, γ-ester, and phenyl glycoside linkages, providing direct evidence of lignin–carbohydrate bond formation in biomimicked lignin polymerization. Based on our findings, we propose a sequence for lignin–carbohydrate bond formation in plant cell walls.

Place, publisher, year, edition, pages
2019.
Keywords [en]
Benzyl ester, benzyl ether, dehydrogenation polymer, xtracellular lignin, gamma (γ)-ester, lignin–carbohydrate complex, phenyl glycoside
National Category
Other Natural Sciences Wood Science
Research subject
Fibre and Polymer Science
Identifiers
URN: urn:nbn:se:kth:diva-258726DOI: 10.1093/jxb/erz324Scopus ID: 2-s2.0-85074118297OAI: oai:DiVA.org:kth-258726DiVA, id: diva2:1350108
Funder
Knut and Alice Wallenberg Foundation, 8107
Note

QC 20191105

Available from: 2019-09-10 Created: 2019-09-10 Last updated: 2019-11-05Bibliographically approved

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Publisher's full textScopushttps://academic.oup.com/jxb/advance-article/doi/10.1093/jxb/erz324/5531090

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Giummarella, NicolaLawoko, Martin

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