Endre søk
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf
Metabolic engineering applications of the Escherichia coli bacterial artificial chromosome
KTH, Skolan för kemi, bioteknologi och hälsa (CBH), Industriell bioteknologi.ORCID-id: 0000-0002-7916-4731
KTH, Skolan för kemi, bioteknologi och hälsa (CBH), Industriell bioteknologi. Univ Mayor San Simon, Ctr Biotechnol, Fac Sci & Technol, Cochabamba, Bolivia..ORCID-id: 0000-0001-6501-9886
KTH, Skolan för kemi, bioteknologi och hälsa (CBH), Industriell bioteknologi.ORCID-id: 0000-0001-5319-7511
KTH, Skolan för kemi, bioteknologi och hälsa (CBH), Industriell bioteknologi.ORCID-id: 0000-0002-3314-6060
2019 (engelsk)Inngår i: Journal of Biotechnology, ISSN 0168-1656, E-ISSN 1873-4863, Vol. 305, s. 43-50Artikkel i tidsskrift (Fagfellevurdert) Published
Abstract [en]

In metabolic engineering and synthetic biology, the number of genes expressed to achieve better production and pathway regulation in each strain is steadily increasing. The method of choice for expression in Escherichia coli is usually one or several multi-copy plasmids. Meanwhile, the industry standard for long-term, robust production is chromosomal integration of the desired genes. Despite recent advances, genetic manipulation of the bacterial chromosome remains more time consuming than plasmid construction. To allow screening of different metabolic engineering strategies at a level closer to industry while maintaining the molecular-biology advantages of plasmid-based expression, we have investigated the single-copy bacterial artificial chromosome (BAC) as a development tool for metabolic engineering. Using (R)-3-hydroxybutyrate as a model product, we show that BAC can outperform multi-copy plasmids in terms of yield, productivity and specific growth rate, with respective increases of 12%, 18%, and 5%. We both show that gene expression by the BAC simplifies pathway optimization and that the phenotype of pathway expression from BAC is very close to that of chromosomal expression. From these results, we conclude that the BAC can provide a simple platform for performing pathway design and optimization.

sted, utgiver, år, opplag, sider
ELSEVIER , 2019. Vol. 305, s. 43-50
Emneord [en]
3-Hydroxybutyrate, Chromosomal expression, Pathway optimization, F plasmid, Bacterial artificial chromosome, Metabolic engineering
HSV kategori
Forskningsprogram
Bioteknologi
Identifikatorer
URN: urn:nbn:se:kth:diva-261932DOI: 10.1016/j.jbiotec.2019.09.002ISI: 000487564300007PubMedID: 31505217Scopus ID: 2-s2.0-85072060887OAI: oai:DiVA.org:kth-261932DiVA, id: diva2:1361261
Merknad

QC 20191015

Tilgjengelig fra: 2019-10-15 Laget: 2019-10-15 Sist oppdatert: 2019-12-02bibliografisk kontrollert

Open Access i DiVA

Fulltekst mangler i DiVA

Andre lenker

Forlagets fulltekstPubMedScopus

Personposter BETA

Sjöberg, GustavGuevara-Martínez, Mónicavan Maris, Antonius J. A.Gustavsson, Martin

Søk i DiVA

Av forfatter/redaktør
Sjöberg, GustavGuevara-Martínez, Mónicavan Maris, Antonius J. A.Gustavsson, Martin
Av organisasjonen
I samme tidsskrift
Journal of Biotechnology

Søk utenfor DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric

doi
pubmed
urn-nbn
Totalt: 12 treff
RefereraExporteraLink to record
Permanent link

Direct link
Referera
Referensformat
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Annet format
Fler format
Språk
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Annet språk
Fler språk
Utmatningsformat
  • html
  • text
  • asciidoc
  • rtf