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Layer by layer coating for bio-functionalization of additively manufactured meta-biomaterials
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2020 (Engelska)Ingår i: Additive Manufacturing, ISSN 2214-8604, Vol. 32Artikel i tidskrift (Refereegranskat) Published
Abstract [en]

Additive manufacturing has facilitated fabrication of complex and patient-specific metallic meta-biomaterials that offer an unprecedented collection of mechanical, mass transport, and biological properties as well as a fully interconnected porous structure. However, applying meta-biomaterials for addressing unmet clinical needs in orthopedic surgery requires additional surface functionalities that should be induced through tailor-made coatings. Here, we developed multi-functional layer-by-layer coatings to simultaneously prevent implant-associated infections and stimulate bone tissue regeneration. We applied multiple layers of gelatin- and chitosan-based coatings containing either bone morphogenetic protein (BMP)-2 or vancomycin on the surface of selective laser melted porous structures made from commercial pure Titanium (CP Ti) and designed using a triply periodic minimal surface (i.e., sheet gyroid). The additive manufacturing process resulted in a porous structure and met the the design values comparatively. X-ray photoelectron spectroscopy spectra confirmed the presence and composition of the coating layers. The release profiles showed a continued release of both vancomycin and BMP-2 for 2–3 weeks. Furthermore, the developed meta-biomaterials exhibited a very strong antibacterial behavior with up to 8 orders of magnitude reduction in both planktonic and implant-adherent bacteria and no signs of biofilm formation. The osteogenic differentiation of mesenchymal stem cells was enhanced, as shown by two-fold increase in the alkaline phosphatase activity and up to four-fold increase in the mineralization of all experimental groups containing BMP-2. Eight-week subcutaneous implantation in vivo showed no signs of a foreign body response, while connective tissue ingrowth was promoted by the layer-by-layer coating. These results unequivocally confirm the superior multi-functional performance of the developed biomaterials.

Ort, förlag, år, upplaga, sidor
2020. Vol. 32
Nyckelord [en]
Meta-biomaterials, Metal 3D printing, Layer-by-layer surface bio-functionalization, Multi-functional coatings
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Teknik och teknologier
Identifikatorer
URN: urn:nbn:se:kth:diva-266330DOI: 10.1016/j.addma.2019.100991Scopus ID: 2-s2.0-85077319545OAI: oai:DiVA.org:kth-266330DiVA, id: diva2:1382992
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QC 20200108

Tillgänglig från: 2020-01-07 Skapad: 2020-01-07 Senast uppdaterad: 2020-03-09Bibliografiskt granskad

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Förlagets fulltextScopushttp://www.sciencedirect.com/science/article/pii/S2214860419314824

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Dadbakhsh, S.
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Skolan för industriell teknik och management (ITM)
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