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Benchmarking virus concentration methods for quantification of SARS-CoV-2 in raw wastewater
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Protein Science. KTH, Centres, Science for Life Laboratory, SciLifeLab.
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Chemical Engineering.
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Chemical Engineering, Resource recovery.ORCID iD: 0000-0003-4046-1592
KTH, Centres, Science for Life Laboratory, SciLifeLab. KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Protein Science. Department of Biosciences and Nutrition, Karolinska Institute, Huddinge, Sweden.ORCID iD: 0000-0002-5876-0710
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2021 (English)In: Science of the Total Environment, ISSN 0048-9697, E-ISSN 1879-1026, Vol. 755Article in journal (Refereed) Published
Abstract [en]

Wastewater-based epidemiology offers a cost-effective alternative to testing large populations for SARS-CoV-2 virus, and may potentially be used as an early warning system for SARS-CoV-2 pandemic spread. However, viruses are highly diluted in wastewater, and a validated method for their concentration and further processing, and suitable reference viruses, are the main needs to be established for reliable SARS-CoV-2 municipal wastewater detection. For this purpose, we collected wastewater from two European cities during the Covid-19 pandemic and evaluated the sensitivity of RT-qPCR detection of viral RNA after four concentration methods (two variants of ultrafiltration-based method and two adsorption and extraction-based methods). Further, we evaluated one external (bovine corona virus) and one internal (pepper mild mottle virus) reference virus. We found a consistently higher recovery of spiked virus using the modified ultrafiltration-based method. This method also had a significantly higher efficiency (p-value <0.01) for wastewater SARS-CoV-2 detection. The ultracentrifugation method was the only method that detected SARS-CoV-2 in the wastewater of both cities. The pepper mild mottle virus was found to function as a potentially suitable internal reference standard.

Place, publisher, year, edition, pages
Elsevier BV , 2021. Vol. 755
Keywords [en]
SARS-CoV-2Bovine corona virusPepper mild mottle virusVirus concentration methodMunicipal wastewaterRT-qPCR
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Other Environmental Biotechnology
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URN: urn:nbn:se:kth:diva-287568DOI: 10.1016/j.scitotenv.2020.142939ISI: 000600537400008PubMedID: 33121776Scopus ID: 2-s2.0-85093951320OAI: oai:DiVA.org:kth-287568DiVA, id: diva2:1510324
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QC 20201216

Available from: 2020-12-15 Created: 2020-12-15 Last updated: 2024-03-18Bibliographically approved

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Hakim Jaffer Ali, MohammedKhatami, KasraAtasoy, MerveBirgersson, MadeleineWilliams, CeciliaCetecioglu, Zeynep

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Hakim Jaffer Ali, MohammedKhatami, KasraAtasoy, MerveBirgersson, MadeleineWilliams, CeciliaCetecioglu, Zeynep
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Protein ScienceScience for Life Laboratory, SciLifeLabChemical EngineeringResource recovery
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