kth.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Optotracing for selective fluorescence-based detection, visualization and quantification of live S. aureus in real-time
Department of Neuroscience, Karolinska Institutet, SE-171 77, Stockholm, Sweden.
Department of Neuroscience, Karolinska Institutet, SE-171 77, Stockholm, Sweden.
Department of Neuroscience, Karolinska Institutet, SE-171 77, Stockholm, Sweden.
Department of Chemistry, IFM, Linköping University, SE-581 83, Linköping, Sweden.
Show others and affiliations
2020 (English)In: npj Biofilms and Microbiomes, E-ISSN 2055-5008, Vol. 6, no 1, article id 35Article in journal (Refereed) Published
Abstract [en]

Methods for bacterial detection are needed to advance the infection research and diagnostics. Based on conformation-sensitive fluorescent tracer molecules, optotracing was recently established for dynamic detection and visualization of structural amyloids and polysaccharides in the biofilm matrix of gram-negative bacteria. Here, we extend the use of optotracing for detection of gram-positive bacteria, focussing on the clinically relevant opportunistic human pathogen Staphylococcus aureus. We identify a donor-acceptor-donor-type optotracer, whose binding-induced fluorescence enables real-time detection, quantification, and visualization of S. aureus in monoculture and when mixed with gram-negative Salmonella Enteritidis. An algorithm-based automated high-throughput screen of 1920 S. aureus transposon mutants recognized the cell envelope as the binding target, which was corroborated by super-resolution microscopy of bacterial cells and spectroscopic analysis of purified cell wall components. The binding event was essentially governed by hydrophobic interactions, which permitted custom-designed tuning of the binding selectivity towards S. aureus versus Enterococcus faecalis by appropriate selection of buffer conditions. Collectively this work demonstrates optotracing as an enabling technology relevant for any field of basic and applied research, where visualization and detection of S. aureus is needed.

Place, publisher, year, edition, pages
Nature Research , 2020. Vol. 6, no 1, article id 35
National Category
Microbiology in the medical area
Identifiers
URN: urn:nbn:se:kth:diva-287876DOI: 10.1038/s41522-020-00150-yISI: 000581660500001PubMedID: 33037198Scopus ID: 2-s2.0-85092296090OAI: oai:DiVA.org:kth-287876DiVA, id: diva2:1513533
Note

QC 20201230

Author correction in: 10.1038/s41522-020-00179-z

Available from: 2020-12-30 Created: 2020-12-30 Last updated: 2022-06-25Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMedScopus

Authority records

Richter-Dahlfors, Agneta

Search in DiVA

By author/editor
Richter-Dahlfors, Agneta
By organisation
School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH)
In the same journal
npj Biofilms and Microbiomes
Microbiology in the medical area

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 11 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf