kth.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Endochondral Ossification Induced by Cell Transplantation of Endothelial Cells and Bone Marrow Stromal Cells with Copolymer Scaffold Using a Rat Calvarial Defect Model
Lanzhou Univ, Sch Stomatol, Lanzhou 730000, Peoples R China.;Univ Bergen, Dept Clin Dent, Fac Med, N-5009 Bergen, Norway..
Lanzhou Univ, Sch Stomatol, Lanzhou 730000, Peoples R China..
Lanzhou Univ, Sch Stomatol, Lanzhou 730000, Peoples R China..
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Fibre- and Polymer Technology, Polymer Technology.ORCID iD: 0000-0002-1922-128X
Show others and affiliations
2021 (English)In: Polymers, E-ISSN 2073-4360, Vol. 13, no 9, article id 1521Article in journal (Refereed) Published
Abstract [en]

It has been recently reported that, in a rat calvarial defect model, adding endothelial cells (ECs) to a culture of bone marrow stromal cells (BMSCs) significantly enhanced bone formation. The aim of this study is to further investigate the ossification process of newly formed osteoid and host response to the poly(L-lactide-co-1,5-dioxepan-2-one) [poly(LLA-co-DXO)] scaffolds based on previous research. Several different histological methods and a PCR Array were applied to evaluate newly formed osteoid after 8 weeks after implantation. Histological results showed osteoid formed in rat calvarial defects and endochondral ossification-related genes, such as dentin matrix acidic phosphoprotein 1 (Dmp1) and collagen type II, and alpha 1 (Col2a1) exhibited greater expression in the CO (implantation with BMSC/EC/Scaffold constructs) than the BMSC group (implantation with BMSC/Scaffold constructs) as demonstrated by PCR Array. It was important to notice that cartilage-like tissue formed in the pores of the copolymer scaffolds. In addition, multinucleated giant cells (MNGCs) were observed surrounding the scaffold fragments. It was concluded that the mechanism of ossification might be an endochondral ossification process when the copolymer scaffolds loaded with co-cultured ECs/BMSCs were implanted into rat calvarial defects. MNGCs were induced by the poly(LLA-co-DXO) scaffolds after implantation, and more specific in vivo studies are needed to gain a better understanding of host response to copolymer scaffolds.

Place, publisher, year, edition, pages
MDPI , 2021. Vol. 13, no 9, article id 1521
Keywords [en]
bone marrow stromal cells, endothelial cells, copolymer scaffold, endochondral ossification, host response
National Category
Biomaterials Science
Identifiers
URN: urn:nbn:se:kth:diva-296195DOI: 10.3390/polym13091521ISI: 000650714000001PubMedID: 34065081Scopus ID: 2-s2.0-85106598397OAI: oai:DiVA.org:kth-296195DiVA, id: diva2:1562860
Note

QC 20210609

Available from: 2021-06-09 Created: 2021-06-09 Last updated: 2024-01-17Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMedScopus

Authority records

Finne Wistrand, Anna

Search in DiVA

By author/editor
Finne Wistrand, Anna
By organisation
Polymer Technology
In the same journal
Polymers
Biomaterials Science

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 62 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf