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Assembly of a Rieske non-heme iron oxygenase multicomponent system from Phenylobacterium immobile E DSM 1986 enables pyrazon cis-dihydroxylation in E. coli
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2021 (English)In: Applied Microbiology and Biotechnology, ISSN 0175-7598, E-ISSN 1432-0614, Vol. 105, no 5, p. 2003-2015Article in journal (Refereed) Published
Abstract [en]

Abstract: Phenylobacterium immobile strain E is a soil bacterium with a striking metabolism relying on xenobiotics, such as the herbicide pyrazon, as sole carbon source instead of more bioavailable molecules. Pyrazon is a heterocyclic aromatic compound of environmental concern and its biodegradation pathway has only been reported in P. immobile. The multicomponent pyrazon oxygenase (PPO), a Rieske non-heme iron oxygenase, incorporates molecular oxygen at the 2,3 position of the pyrazon phenyl moiety as first step of degradation, generating a cis-dihydrodiendiol. The aim of this work was to identify the genes encoding for each one of the PPO components and enable their functional assembly in Escherichia coli. P. immobile strain E genome sequencing revealed genes encoding for RO components, such as ferredoxin-, reductase-, α- and β-subunits of an oxygenase. Though, P. immobile E displays three prominent differences with respect to the ROs currently characterized: (1) an operon-like organization for PPO is absent, (2) all the elements are randomly scattered in its DNA, (3) not only one, but 19 different α-subunits are encoded in its genome. Herein, we report the identification of the PPO components involved in pyrazon cis-dihydroxylation in P. immobile, its appropriate assembly, and its functional reconstitution in E. coli. Our results contributes with the essential missing pieces to complete the overall elucidation of the PPO from P. immobile. Key points: • Phenylobacterium immobile E DSM 1986 harbors the only described pyrazon oxygenase (PPO). • We elucidated the genes encoding for all PPO components. • Heterologous expression of PPO enabled pyrazon dihydroxylation in E. coli JW5510. 

Place, publisher, year, edition, pages
Springer Science and Business Media Deutschland GmbH , 2021. Vol. 105, no 5, p. 2003-2015
Keywords [en]
Biocatalysis, Biodegradation, Cis-dihydroxylation, Phenylobacterium immobile, Pyrazon oxygenase, Rieske non-heme iron oxygenases, Aromatic compounds, Biochemistry, Encoding (symbols), Genes, Hydroxylation, Iron, Molecular oxygen, Porphyrins, Signal encoding, Biodegradation pathways, Environmental concerns, Genome sequencing, Heterocyclic aromatic compounds, Heterologous expression, Multi-component systems, Multicomponents, Sole carbon source, Escherichia coli, ampicillin, bacterial DNA, chloridazon, ferredoxin, oxidoreductase, oxygenase, rieske non heme iron oxygenase, unclassified drug, pyridazine derivative, bacterium, bioavailability, chemical compound, chemical reaction, coliform bacterium, detection method, enzyme, enzyme activity, gene expression, genome, microbial activity, alpha chain, Article, bacterial genome, bacterial strain, beta chain, controlled study, degradation, dihydroxylation, gene identification, gene sequence, genetic code, nonhuman, Phenylobacterium immobile e dsm 1986, soil microflora, Caulobacteraceae, genetics, Bacteria (microorganisms), Oxygenases, Pyridazines
National Category
Microbiology Microbiology in the medical area Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:kth:diva-305490DOI: 10.1007/s00253-021-11129-wISI: 000617837100001PubMedID: 33582834Scopus ID: 2-s2.0-85100842021OAI: oai:DiVA.org:kth-305490DiVA, id: diva2:1615442
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QC 20211130

Available from: 2021-11-30 Created: 2021-11-30 Last updated: 2022-06-25Bibliographically approved

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Syrén, Per-Olof

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