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Regulation of colony morphology and biofilm formation in Shewanella algae
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden.
Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden.
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Chemistry, Glycoscience.ORCID iD: 0000-0002-0806-1989
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Chemistry, Glycoscience.ORCID iD: 0000-0003-3572-7798
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2021 (English)In: Microbial Biotechnology, ISSN 1751-7907, E-ISSN 1751-7915, Vol. 14, no 3, p. 1183-1200Article in journal (Refereed) Published
Abstract [en]

Bacterial colony morphology can reflect different physiological stages such as virulence or biofilm formation. In this work we used transposon mutagenesis to identify genes that alter colony morphology and cause differential Congo Red (CR) and Brilliant Blue G (BBG) binding in Shewanella algae, a marine indigenous bacterium and occasional human pathogen. Microscopic analysis of colonies formed by the wild-type strain S. algae CECT 5071 and three transposon integration mutants representing the diversity of colony morphotypes showed production of biofilm extracellular polymeric substances (EPS) and distinctive morphological alterations. Electrophoretic and chemical analyses of extracted EPS showed differential patterns between strains, although the targets of CR and BBG binding remain to be identified. Galactose and galactosamine were the preponderant sugars in the colony biofilm EPS of S. algae. Surface-associated biofilm formation of transposon integration mutants was not directly correlated with a distinct colony morphotype. The hybrid sensor histidine kinase BarA abrogated surface-associated biofilm formation. Ectopic expression of the kinase and mutants in the phosphorelay cascade partially recovered biofilm formation. Altogether, this work provides the basic analysis to subsequently address the complex and intertwined networks regulating colony morphology and biofilm formation in this poorly understood species.

Place, publisher, year, edition, pages
Wiley , 2021. Vol. 14, no 3, p. 1183-1200
Keywords [en]
agar, brilliant blue g, congo red, galactosamine, galactose, protein BarA, protein histidine kinase, unclassified drug, Article, bacterial growth, bacterial structures, bacterium colony, bacterium culture, biofilm, biofilm matrix, chemical analysis, chemical binding, controlled study, electrophoresis, extracellular matrix, Fourier transform infrared spectroscopy, gene identification, gene interaction, genetic variability, microscopy, morphotype, nonhuman, protein expression, protein function, scanning electron microscopy, Shewanella, transposon mutagenesis, whole genome sequencing, genetics, human, mutagenesis, virulence, Biofilms, Humans
National Category
Microbiology Microbiology in the medical area
Identifiers
URN: urn:nbn:se:kth:diva-307424DOI: 10.1111/1751-7915.13788ISI: 000632453900001PubMedID: 33764668Scopus ID: 2-s2.0-85103052177OAI: oai:DiVA.org:kth-307424DiVA, id: diva2:1631890
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QC 20220216

Available from: 2022-01-25 Created: 2022-01-25 Last updated: 2022-06-25Bibliographically approved

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Yilmaz Turan, SecilVilaplana, Francisco

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