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Synthesis and in Vitro Toxicity Assessment of Different Nano-Calcium Phosphate Nanoparticles
Univ Bayburt, Vocat Sch Hlth Serv, Dept Med Serv & Tech, Bayburt, Turkey..
Univ Ataturk, Sch Med, Dept Med Biol, Erzurum, Turkey..
Univ Sabanci, Nanotechnol Res & Applicat Ctr, Istanbul, Turkey..
Univ Erzurum Tech, Fac Sci, Dept Mol Biol & Genet, Erzurum, Turkey..
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2022 (English)In: Brazilian archives of biology and technology, ISSN 1516-8913, E-ISSN 1678-4324, Vol. 65, article id e22200784Article in journal (Refereed) Published
Abstract [en]

Nanoscale biomaterials are commonly used in a wide range of biomedical applications such as bone graft substitutes, gene delivery systems, and biologically active agents. On the other hand, the cytotoxic potential of these particles hasn't yet been studied comprehensively to understand whether or not they exert any negative impact on the cellular structures. Here, we undertook the synthesis of beta-tricalcium phosphate (beta-TCP) and biphasic tricalcium phosphate (BCP) nanoparticles (NPs) and determine their concentration-dependent toxic effects in human fetal osteoblastic (hFOB 1.19) cell line. Firstly, BCP and beta-TCP were synthesized using a water-based precipitation technique and characterized by X-Ray Diffraction (XRD), Raman Spectroscopy, and Transmission Electron Microscopy (TEM). The cytological effects of beta-TCP and BCP at different concentrations (0-640 ppm) were evaluated by using 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assays. The total oxidative status (TOS) parameter was used for investigating oxidative stress potentials of the NPs. In addition, the study assessed the DNA damage product 8-hydroxy-2'-deoxyguanosine (8-Oxo-dG) level in hFOB 1.19 cell cultures. The results indicated that the beta-TCP (above 320 ppm) and BCP (above 80 ppm) NPs exhibited cytotoxicity effects on high concentrations. It was also observed that the oxidative stress increased relatively as the concentrations of NPs increased, aligning with the cytotoxicity results. However, the NPs concentrations of 160 ppm and above increased the level of 8-OH-dG. Consequently, there is a need for more systematic in vivo and in vitro approaches to the toxic effects of both nanoparticles.

Place, publisher, year, edition, pages
FapUNIFESP (SciELO) , 2022. Vol. 65, article id e22200784
Keywords [en]
beta-Tricalcium phosphate, biphasic calcium phosphate, nanoparticle, cytotoxicity, total oxidative status, genotoxicity
National Category
Dentistry Orthopaedics
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URN: urn:nbn:se:kth:diva-310646DOI: 10.1590/1678-4324-2022200784ISI: 000768802200001Scopus ID: 2-s2.0-85130055817OAI: oai:DiVA.org:kth-310646DiVA, id: diva2:1650206
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QC 20220406

Available from: 2022-04-06 Created: 2022-04-06 Last updated: 2023-06-08Bibliographically approved

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Mardinoglu, Adil

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