kth.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
RNAlater som bevaringsmetod för muskelvävnad: En jämförande metodstudie om frystorkning, RNAlater och RNAlater-ICE som bevaring av skelettmuskulatur inför molekylärbiologiska analyser
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Biomedical Engineering and Health Systems.
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Biomedical Engineering and Health Systems.
2022 (Swedish)Independent thesis Basic level (degree of Bachelor), 10 credits / 15 HE creditsStudent thesisAlternative title
RNAlater as a Method for Preservation of Muscle Tissue : A Comparative Method Study on Lyophilization, RNAlater and RNAlater ICE for Preservation of Human Skeletal Muscle Preceding Molecular Biological Analyzes (English)
Abstract [sv]

I denna metodstudie jämfördes Thermo Fishers bevarande lösningar RNAlater och RNAlater-ICE med frystorkning som bevaring av skelettmuskulatur från människa. Studien gjordes med syftet att avgöra om RNAlater och/eller RNAlater-ICE kan ersätta frystorkning, i hopp om att underlätta dissekering av muskelvävnad. De molekylärbiologiska analyser som utfördes var proteinbestämning; Western Blot inriktad på proteinerna mTor, S6, S6K1, eEF2, myosin typ II och aktin; samt mätning av glykogen och citratsyntasaktivitet. Därtill påbörjades även en aminosyraanalys. Studien utfördes hos William Apró på Åstrandlaboratoriet på Gymnastik- och idrottshögskolan i Stockholm. Resultaten visade att både RNAlater och RNAlater-ICE är olämpliga att använda i studier som innefattar samtliga av de genomförda analyserna. Detta då analysresultaten från de alternativa bevaringsmetoderna och de från frystorkningen inte var likvärdiga. Därmed drogs slutsatsen att varken RNAlater eller RNAlater-ICE kan ersätta frystorkning som bevaringsmetod i Aprós vidare studier.

Abstract [en]

In this method study Thermo Fisher’s preserving solutions RNAlater and RNAlater-ICE were compared to lyophilization for preservation of human skeletal muscle. The study was conducted with the aim of determining whether RNAlater and / or RNAlater-ICE can replace lyophilization, in the hope of facilitating dissection of muscle tissue. The molecular biological analyzes performed were protein assay; Western Blot focused on the proteins mTor, S6, S6K1, eEF2, myosin type II and actin; alongside of measurments on glycogen and citrate synthase activity. In addition, an amino acid analysis was initiated. The study was executed with William Apró at the Åstrand Laboratory at The Swedish School of Sport and Health Science in Stockholm. The results showed that both RNAlater and RNAlater-ICE are unsuitable for use in studies that include all of the analyzes performed. This is because the analysis results from the alternative preservation methods and those from lyophilization were not equivalent. Thus, it was concluded that neither RNAlater nor RNAlater-ICE can replace lyophilization as a preservation method in Apró’s further studies.

Place, publisher, year, edition, pages
2022. , p. 52
Series
TRITA-CBH-GRU ; 2022:146
Keywords [sv]
RNAlater, RNAlater-ICE, frystorkning, bevaringsmetod, skelettmuskulatur, muskelfibrer, protein, Western Blot, proteinbestämning, glykogenmängd, enzymaktivitet, molekylärbiologi
National Category
Biomedical Laboratory Science/Technology
Identifiers
URN: urn:nbn:se:kth:diva-315312OAI: oai:DiVA.org:kth-315312DiVA, id: diva2:1679874
External cooperation
Gymnastik- och idrottshögskolan
Subject / course
Medical Engineering
Educational program
Master of Science in Engineering - Medical Engineering
Supervisors
Examiners
Available from: 2022-07-05 Created: 2022-07-01 Last updated: 2022-07-05Bibliographically approved

Open Access in DiVA

fulltext(14456 kB)109 downloads
File information
File name FULLTEXT01.pdfFile size 14456 kBChecksum SHA-512
c680e959c9c9aee7ad41ff7bfed40beca86da1d23e2e8a540ef3f151ab536c67dc0257f6218e9b1322d4bc3e0557bbd2d7580087bfe2a1fa4b31d0efdae95c66
Type fulltextMimetype application/pdf

By organisation
Biomedical Engineering and Health Systems
Biomedical Laboratory Science/Technology

Search outside of DiVA

GoogleGoogle Scholar
Total: 109 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

urn-nbn

Altmetric score

urn-nbn
Total: 420 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf