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An optotracer-based antibiotic susceptibility test specifically targeting the biofilm lifestyle of Salmonella
Karolinska Inst, AIMES Ctr Advancement Integrated Med & Engn Sci, Stockholm, Sweden.;Karolinska Inst, Dept Neurosci, Solnavagen 9, SE-17177 Stockholm, Sweden.;Swiss Fed Inst Technol, Dept Biol, Zurich, Switzerland..
Karolinska Inst, AIMES Ctr Advancement Integrated Med & Engn Sci, Stockholm, Sweden.;KTH Royal Inst Technol, Stockholm, Sweden.;Karolinska Inst, Dept Neurosci, Solnavagen 9, SE-17177 Stockholm, Sweden..
Karolinska Inst, AIMES Ctr Advancement Integrated Med & Engn Sci, Stockholm, Sweden.;KTH Royal Inst Technol, Stockholm, Sweden.;Karolinska Inst, Dept Microbiol Tumor & Cell Biol, Solnavagen 9, SE-17177 Stockholm, Sweden..
Karolinska Inst, AIMES Ctr Advancement Integrated Med & Engn Sci, Stockholm, Sweden.;KTH Royal Inst Technol, Stockholm, Sweden.;Karolinska Inst, Dept Neurosci, Solnavagen 9, SE-17177 Stockholm, Sweden..
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2022 (English)In: Biofilm, E-ISSN 2590-2075, Vol. 4, p. 100083-, article id 100083Article in journal (Refereed) Published
Abstract [en]

Antimicrobial resistance is a medical threat of global dimensions. Proper antimicrobial susceptibility testing (AST) for drug development, patient diagnosis and treatment is crucial to counteract ineffective drug use and resistance development. Despite the important role of bacterial biofilms in chronic and device-associated in-fections, the efficacy of antibiotics is determined using planktonic cultures. To address the need for antibiotics targeting bacteria in the biofilm lifestyle, we here present an optotracing-based biofilm-AST using Salmonella as model. Our non-disruptive method enables real-time recording of the extracellular matrix (ECM) components, providing specific detection of the biofilm lifestyle. Biofilm formation prior to antibiotic challenge can thus be confirmed and pre-treatment data collected. By introducing Kirby-Bauer discs, we performed a broad screen of the effects of antibiotics representing multiple classes, and identified compounds with ECM inhibitory as well as promoting effects. These compounds were further tested in agar-based dose-response biofilm-AST assays. By quantifying the ECM based on the amount of curli, and by visualizing the biofilm size and morphology, we achieved new information directly reflecting the treated biofilm. This verified the efficacy of several antibiotics that were effective in eradicating pre-formed biofilms, and it uncovered intriguing possible resistance mecha-nisms initiated in response to treatments. By providing deeper insights into the resistances and susceptibilities of microbes, expanded use of the biofilm-AST will contribute to more effective treatments of infections and reduced resistance development.

Place, publisher, year, edition, pages
Elsevier BV , 2022. Vol. 4, p. 100083-, article id 100083
Keywords [en]
Salmonella biofilm, ECM, Curli, Optotracing, EbbaBiolight 680, Real-time, AST
National Category
Microbiology in the medical area
Identifiers
URN: urn:nbn:se:kth:diva-321296DOI: 10.1016/j.bioflm.2022.100083ISI: 000874897100001PubMedID: 36117547Scopus ID: 2-s2.0-85137664397OAI: oai:DiVA.org:kth-321296DiVA, id: diva2:1711030
Note

QC 20221115

Available from: 2022-11-15 Created: 2022-11-15 Last updated: 2023-01-25Bibliographically approved

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Richter-Dahlfors, Agneta

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