kth.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
In Vitro Transcriptome Analysis of Cobalt Boride Nanoparticles on Human Pulmonary Alveolar Cells
Erzurum Tech Univ, Fac Sci, Dept Mol Biol & Genet, TR-25050 Erzurum, Turkey..ORCID iD: 0000-0002-1600-2305
Ataturk Univ, Fac Med, Dept Otorhinolaryngol, TR-25240 Erzurum, Turkey..
Erzurum Tech Univ, Fac Sci, Dept Mol Biol & Genet, TR-25050 Erzurum, Turkey..
Ondokuz Mayis Univ, Fac Med, Dept Pediat, Pediat Cardiol, TR-55139 Samsun, Turkey..
Show others and affiliations
2022 (English)In: Materials, ISSN 1996-1944, E-ISSN 1996-1944, Vol. 15, no 23, article id 8683Article in journal (Refereed) Published
Abstract [en]

Nanobiotechnology influences many different areas, including the medical, food, energy, clothing, and cosmetics industries. Considering the wide usage of nanomaterials, it is necessary to investigate the toxicity potentials of specific nanosized molecules. Boron-containing nanoparticles (NPs) are attracting much interest from scientists due to their unique physicochemical properties. However, there is limited information concerning the toxicity of boron-containing NPs, including cobalt boride (Co2B) NPs. Therefore, in this study, Co2B NPs were characterized using X-ray crystallography (XRD), transmission electron microscope (TEM), scanning electron microscope (SEM), and energy-dispersive X-ray spectroscopy (EDX) techniques. Then, we performed 3-(4,5-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium bromide (MTT), lactate dehydrogenase (LDH) release, and neutral red (NR) assays for assessing cell viability against Co2B NP exposure on cultured human pulmonary alveolar epithelial cells (HPAEpiC). In addition, whole-genome microarray analysis was carried out to reveal the global gene expression differentiation of HPAEpiC cells after Co2B NP application. The cell viability tests unveiled an IC50 value for Co2B NPs of 310.353 mg/L. The results of our microarray analysis displayed 719 gene expression differentiations (FC >= 2) among the analyzed 40,000 genes. The performed visualization and integrated discovery (DAVID) analysis revealed that there were interactions between various gene pathways and administration of the NPs. Based on gene ontology biological processes analysis, we found that the P53 signaling pathway, cell cycle, and cancer-affecting genes were mostly affected by the Co2B NPs. In conclusion, we suggested that Co2B NPs would be a safe and effective nanomolecule for industrial applications, particularly for medical purposes.

Place, publisher, year, edition, pages
MDPI AG , 2022. Vol. 15, no 23, article id 8683
Keywords [en]
cobalt boride nanoparticles, toxicogenomics, human pulmonary alveolar epithelial cells, pathway analysis, in vitro, cytotoxicity
National Category
Microbiology in the medical area
Identifiers
URN: urn:nbn:se:kth:diva-322895DOI: 10.3390/ma15238683ISI: 000896211800001PubMedID: 36500178Scopus ID: 2-s2.0-85143783691OAI: oai:DiVA.org:kth-322895DiVA, id: diva2:1724614
Note

QC 20230109

Available from: 2023-01-09 Created: 2023-01-09 Last updated: 2023-01-09Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMedScopus

Authority records

Mardinoglu, Adil

Search in DiVA

By author/editor
Arslan, Mehmet EnesOzdemir, OzlemMardinoglu, Adil
By organisation
Systems BiologyScience for Life Laboratory, SciLifeLab
In the same journal
Materials
Microbiology in the medical area

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 45 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf