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Engineering Surfaces with Immune Modulating Properties of Mucin Hydrogels
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Chemistry, Glycoscience. KTH, Centres, Center for the Advancement of Integrated Medical and Engineering Sciences, AIMES.ORCID iD: 0000-0003-1525-1227
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Chemistry, Glycoscience.
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Fibre- and Polymer Technology.ORCID iD: 0000-0002-5444-7276
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Chemistry, Glycoscience. KTH, Centres, Center for the Advancement of Integrated Medical and Engineering Sciences, AIMES.ORCID iD: 0000-0002-1981-3736
2022 (English)In: ACS Applied Materials and Interfaces, ISSN 1944-8244, E-ISSN 1944-8252, Vol. 14, no 35, p. 39727-39735Article in journal (Refereed) Published
Abstract [en]

Hydrogels of cross-linked mucin glycoproteins (Muc-gel) have shown strong immune-modulating properties toward macrophages in vitro, which are translated in vivo by the dampening of the foreign body response to implantation in mice. Beyond mucin hydrogels, other biomaterials such as sensors, electrodes, and other long-term implants would also benefit from such immune-modulating properties. In this work, we aimed to transfer the bioactivity observed for three-dimensional Muc-gels to the surface of two model materials by immobilizing mucin into thin films (Muc-film) using covalent layer-by-layer assembly. We tested how the surface immobilization of mucins affects macrophage responses compared to Muc-gels. We showed that Muc-films on soft polyacrylamide gels mimic Muc-gel in their modulation of macrophage responses with activated gene expression of inflammatory cytokines on day 1 and then dampening them on day 3. Also, the markers of polarized macrophages, M1 and M2, were expressed at the same level for macrophages on Muc-film-coated soft polyacrylamide gels and Muc-gel. In contrast, Muc-film-coated hard polystyrene led to a different macrophage response compared to Muc-gel, having no activated expression of inflammatory cytokines and a different M1 marker expression. This suggested that the substrate mechanical properties and mucin molecular configuration determined by substrate-mucin interactions affect mucin immune-modulating properties. We conclude that mucin immune-modulating properties can be transferred to materials by mucin surface immobilization but will be dependent on the substrate chemical and mechanical properties. 

Place, publisher, year, edition, pages
American Chemical Society (ACS) , 2022. Vol. 14, no 35, p. 39727-39735
Keywords [en]
biomaterials, immune-modulating, macrophage polarization, mucin coating, Crosslinking, Gels, Hydrogels, Macrophages, Thin films, Engineering surfaces, In-vitro, Inflammatory cytokines, Polyacrylamide gels, Property, Surface immobilization, Thin-films, Gene expression, biological marker, biomaterial, cytokine, glycoprotein, mucin, mucin 1, animal, chemistry, hydrogel, macrophage, metabolism, mouse, Animals, Biocompatible Materials, Biomarkers, Cytokines, Glycoproteins, Mice, Mucin-1, Mucins
National Category
Biomaterials Science
Identifiers
URN: urn:nbn:se:kth:diva-327045DOI: 10.1021/acsami.1c19250ISI: 000848450400001PubMedID: 36000701Scopus ID: 2-s2.0-85137391655OAI: oai:DiVA.org:kth-327045DiVA, id: diva2:1758493
Note

QC 20230523

Available from: 2023-05-23 Created: 2023-05-23 Last updated: 2023-05-23Bibliographically approved

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Jiang, KunPettersson, TorbjörnCrouzier, Thomas

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GlycoscienceCenter for the Advancement of Integrated Medical and Engineering Sciences, AIMESFibre- and Polymer Technology
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ACS Applied Materials and Interfaces
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