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A tunable decellularized liver-based hybrid bioink
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Protein Science, Nano Biotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.
Biopromic AB, Solna, Sweden.
KTH, School of Engineering Sciences in Chemistry, Biotechnology and Health (CBH), Protein Science, Nano Biotechnology. KTH, Centres, Science for Life Laboratory, SciLifeLab.ORCID iD: 0000-0003-4923-6965
Indian Institute of Technology, Hyderabad, India.
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2021 (English)In: MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Chemical and Biological Microsystems Society , 2021, p. 281-282Conference paper, Published paper (Refereed)
Abstract [en]

Decellularized extracellular matrix is a tissue-specific biomaterial that recapitulates the complexity of the inherent tissue environment to elicit cellular response. Here, a multi-material decellularized liver (dLM)-based bioink with gelatin is developed and polyethylene glycol crosslinking is used to enhance the viscoelasticity of the dLM. We evaluated the necessity of a post-printing secondary cross-linker mushroom tyrosinase to improve robustness and long term stability. We further demonstrate it's biocompatibility using liver specific gene analysis of HepG2 cells.

Place, publisher, year, edition, pages
Chemical and Biological Microsystems Society , 2021. p. 281-282
Keywords [en]
bioink, decellularization, dLM, SVA-PEG-SVA, tyrosinase, viscoelastic
National Category
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
Identifiers
URN: urn:nbn:se:kth:diva-329656Scopus ID: 2-s2.0-85136938552OAI: oai:DiVA.org:kth-329656DiVA, id: diva2:1773494
Conference
25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021, Palm Springs, 10-14 October 2021
Note

Part of ISBN 9781733419031

QC 20230614

Available from: 2023-06-22 Created: 2023-06-22 Last updated: 2023-06-22Bibliographically approved

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Khati, VamakshiGaudenzi, GiuliaSvahn Andersson, HeleneRussom, Aman

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Khati, VamakshiGaudenzi, GiuliaSvahn Andersson, HeleneRussom, Aman
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Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)

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