kth.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Dynamic single cell analysis in a proximal-tubule-on-chip reveals heterogeneous epithelial colonization strategies of uropathogenic Escherichia coli under shear stress
AIMES - Center for the Advancement of Integrated Medical and Engineering Sciences, Karolinska Institutet, Department of Neuroscience, Karolinska Institutet, SE-171 77 Stockholm, Sweden.
AIMES - Center for the Advancement of Integrated Medical and Engineering Sciences, Karolinska Institutet, Department of Neuroscience, Karolinska Institutet, SE-171 77 Stockholm, Sweden.
AIMES - Center for the Advancement of Integrated Medical and Engineering Sciences, Karolinska Institutet, Sweden; Department of Neuroscience, Karolinska Institutet, SE-171 77 Stockholm, Sweden.
AIMES - Center for the Advancement of Integrated Medical and Engineering Sciences, Karolinska Institutet Department of Neuroscience, Karolinska Institutet, SE-171 77 Stockholm, Sweden.
Show others and affiliations
2023 (English)In: FEMS Microbes, E-ISSN 2633-6685, Vol. 4, article id xtad007Article in journal (Refereed) Published
Abstract [en]

The urinary tract is a hydrodynamically challenging microenvironment and uropathogenic Escherichia coli (UPEC) must overcome several physiological challenges in order to adhere and establish a urinary tract infection. Our previous work in vivo revealed a synergy between different UPEC adhesion organelles, which facilitated effective colonization of the renal proximal tubule. To allow highresolution real-time analysis of this colonization behavior, we established a biomimetic proximal-tubule-on-chip (PToC). The PToC allowed for single-cell resolution analysis of the first stages of bacterial interaction with host epithelial cells, under physiological flow. Time-lapse microscopy and single-cell trajectory analysis in the PToC revealed that while the majority of UPEC moved directly through the system, a minority population initiated heterogeneous adhesion, identified as either rolling or bound. Adhesion was predominantly transient andmediated by P pili at the earliest time-points. These bound bacteria initiated a founder populationwhich rapidly divided, leading to 3D microcolonies. Within the first hours, the microcolonies did not express extracellular curli matrix, but rather were dependent on Type 1 fimbriae as the key element in the microcolony structure. Collectively, our results show the application of Organ-on-chip technology to address bacterial adhesion behaviors, demonstrating a well-orchestrated interplay and redundancy between adhesion organelles that enables UPEC to form microcolonies and persist under physiological shear stress.

Place, publisher, year, edition, pages
Oxford University Press (OUP) , 2023. Vol. 4, article id xtad007
Keywords [en]
biofilm, curli, microcolony, microfluidics, optotracing, UPEC
National Category
Microbiology in the medical area Cell and Molecular Biology Microbiology
Identifiers
URN: urn:nbn:se:kth:diva-340271DOI: 10.1093/femsmc/xtad007Scopus ID: 2-s2.0-85177496779OAI: oai:DiVA.org:kth-340271DiVA, id: diva2:1816173
Note

QC 20231201

Available from: 2023-12-01 Created: 2023-12-01 Last updated: 2023-12-01Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textScopus

Authority records

Richter-Dahlfors, Agneta

Search in DiVA

By author/editor
Antypas, HarisZhang, TianqiChoong, Ferdinand X.Melican, KeiraRichter-Dahlfors, Agneta
By organisation
Fibre- and Polymer TechnologyCenter for the Advancement of Integrated Medical and Engineering Sciences, AIMES
Microbiology in the medical areaCell and Molecular BiologyMicrobiology

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 29 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf