kth.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Performance of an innovative culture-based digital dipstick for detection of bacteriuria
KTH, School of Electrical Engineering and Computer Science (EECS), Intelligent systems, Micro and Nanosystems. UTIlizer AB, Stockholm, Sweden.ORCID iD: 0000-0002-1559-3692
Department of Clinical Microbiology, Karolinska University Hospital, Stockholm, Sweden.
BaseClear BV, Leiden, the Netherlands.ORCID iD: 0000-0001-8545-5108
KTH, School of Electrical Engineering and Computer Science (EECS), Intelligent systems, Micro and Nanosystems.ORCID iD: 0000-0001-8248-6670
Show others and affiliations
2023 (English)In: Microbiology Spectrum, E-ISSN 2165-0497Article in journal (Refereed) Published
Abstract [en]

UTI-lizer is a recent digital format for easy-to-use culture-based detection, preliminary identification, and quantification of bacteria in urine at the point of care (PoC). This study aimed to evaluate the diagnostic accuracy of UTI-lizer tests for detection of bacteriuria caused by five common bacterial species: Escherichia coli, Klebsiella pneumoniae, Enterococcus faecalis, Proteus mirabilis, and Staphylococcus saprophyticus. We evaluated the accuracy of UTI-lizer tests by comparing test results of UTI-lizer with those of current standard bacterial culture-based diagnostics in clinical microbiology laboratories in a retrospective and a prospective study. Comparator methods were classical bacterial culture in combination with matrix-assisted laser desorption/ionization-time of flight mass spectrometry mediated bacterial identification. In the retrospective study, we tested 104 urine samples with in-panel microorganisms, plain urethral microbiota, and culture-negative samples. In the prospective study, we used 137 urine samples within 10 hours of their collection at general practitioner clinics. The retrospective study demonstrated 100% sensitivity and specificity in the detection of bacteriuria, and 98.6% sensitivity and 96.8% specificity in identifying primary pathogens with UTI-lizer when compared to clinical standards. S. saprophyticus and E. coli could not be distinguished. The combined nitrite and esterase test predicted the presence of bacteriuria in only 36.5% of cases. The prospective study demonstrated 100% sensitivity and 89.6% specificity in the detection of significant bacteriuria for in-panel microorganisms with a coverage rate of 88.3% (121/137). This study indicates that digital dipsticks are a promising alternative for the detection of the five main pathogens that cause the vast majority of urinary tract infections (UTIs). The results demonstrate that digital dipsticks have the potential to uniquely provide—in primary care or at the point of care—a UTI diagnostic quality on par with that of current gold-standard testing. The ease of testing, rapid test handling time, and time to result as well as simple test equipment make digital dipsticks an attractive solution for decentralized testing for bacteriuria and with that improvement in UTI diagnostics. These results motivate future studies to validate the use of UTI-lizer at the PoC setting.

Place, publisher, year, edition, pages
American Society for Microbiology , 2023.
National Category
Microbiology in the medical area
Identifiers
URN: urn:nbn:se:kth:diva-341016DOI: 10.1128/spectrum.03613-23ISI: 001125617400004PubMedID: 38088544Scopus ID: 2-s2.0-85182501701OAI: oai:DiVA.org:kth-341016DiVA, id: diva2:1820487
Funder
Vinnova, 2022-01431Vinnova, 2022-02297
Note

QC 20231218

Available from: 2023-12-18 Created: 2023-12-18 Last updated: 2024-01-25Bibliographically approved

Open Access in DiVA

No full text in DiVA

Other links

Publisher's full textPubMedScopus

Authority records

Iseri, Emrevan der Wijngaart, Wouter

Search in DiVA

By author/editor
Iseri, Emrevan Belkum, Alexvan der Wijngaart, WouterÖzenci, Volkan
By organisation
Micro and Nanosystems
In the same journal
Microbiology Spectrum
Microbiology in the medical area

Search outside of DiVA

GoogleGoogle Scholar

doi
pubmed
urn-nbn

Altmetric score

doi
pubmed
urn-nbn
Total: 44 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf